リコンビナントRabMAb

Anti-USP16 抗体 [EPR20914-47] - BSA and Azide free (ab269961)

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Western blot - Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)
  • Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20914-47] to USP16 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat

製品の概要

  • 製品名

    Anti-USP16 antibody [EPR20914-47] - BSA and Azide free
    USP16 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20914-47] to USP16 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Rat testis and liver lysates; Mouse testis lysate; MEF whole cell lysate.

  • 特記事項

    ab269961 is the carrier-free version of ab236628. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
WB
Rat
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 94 kDa).
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 94 kDa).
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.

    • ターゲット情報

    • 機能

      Specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.

    • 組織特異性

      Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

    • 関連疾患

      Note=A chromosomal aberration involving USP16 is a cause of Chronic myelomonocytic leukemia. Inversion inv(21) (q21;q22) with RUNX1/AML1.

    • 配列類似性

      Belongs to the peptidase C19 family. USP16 subfamily.
      Contains 1 UBP-type zinc finger.

    • ドメイン

      The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.

    • 翻訳後修飾

      Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. The phosphorylated form of the protein is also enzymatically active.

    • 細胞内局在

      Nucleus.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Deubiquitinating enzyme 16 antibody
        • Human ubiquitin processing protease antibody
        • MSTP039 antibody
        • Ubiquitin carboxyl-terminal hydrolase 16 antibody
        • Ubiquitin Specific peptidase 16 antibody
        • Ubiquitin Specific Protease 16 antibody
        • Ubiquitin thiolesterase 16 antibody
        • Ubiquitin-processing protease UBP-M antibody
        • Ubiquitin-specific-processing protease 16 antibody
        • UBP M antibody
        • UBP16_HUMAN antibody
        • UBPM antibody
        • usp16 antibody
        see all

      画像

      • Western blot - Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)
        Western blot - Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)
        All lanes : Anti-USP16 antibody [EPR20914-47] (ab236628) at 1/1000 dilution

        Lane 1 : Rat testis tissue lysate at 20 µg
        Lane 2 : Rat liver tissue lysate at 20 µg
        Lane 3 : Mouse testis tissue lysate at 20 µg
        Lane 4 : MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate at 10 µg

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 94 kDa
        Observed band size: 110 kDa why is the actual band size different from the predicted?



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        The molecular weight observed is consistent with what has been described in the literature (PMID: 26323689). 

        Exposure time: Lanes 1-3: 3 minutes Lane 4: 48 seconds

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab236628).

      • Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)
        Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab269961 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab269961 は論文での使用が確認できていません。

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