Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit - Direct Assay (ab108916)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Plasma, Serum
- Sensitivity: 0.062 IU/ml
製品の概要
-
製品名
Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit - Direct Assay
Urokinase キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Enzyme activity (quantitative) -
検出感度
< 0.062 IU/ml -
種交差性
交差種: Human -
製品の概要
Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodeling in angiogeenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.
ab108916 uPA Human Chromogenic Activity Assay Kit is developed to determine Human uPA activity in plasma, serum and cell culture supernatants. The amidolytic activity of uPA is quantitated using a highly specific uPA substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the uPA enzymatic activity.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
試験プラットフォーム
Microplate reader
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 96 tests Assay Diluent 1 x 30ml Human uPA Standard 1 vial Microplate 1 unit Sealing Tapes 3 units uPA Substrate 3 units -
研究分野
-
機能
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
組織特異性
Expressed in the prostate gland and prostate cancers. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
翻訳後修飾
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- ATF
- ATF uPA
- BDPLT5
see all -
参照データベース
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- Unigene: 77274 Human
画像
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab108916 は 1 報の論文で使用されています。
- Matsuyama A et al. Prognostic significance of the urokinase plasminogen activator system in tissue and serum of dogs with appendicular osteosarcoma. PLoS One 17:e0273811 (2022). PubMed: 36174075