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Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit (ab108915)

Submit a review Q&A (2)References (2)
Indirect ELISA - Urokinase type plasminogen activator Human Chromogenic Activity Assay Kit (ab108915)
  • Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)
  • Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Cell culture supernatant, Plasma
  • Sensitivity: 0.0053 U/ml

製品の概要

  • 製品名

    Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit
    Urokinase キット 製品一覧

  • 検出方法

    Colorimetric

  • サンプルの種類

    Cell culture supernatant, Plasma

  • アッセイタイプ

    Enzyme activity (quantitative)

  • 検出感度

    0.0026 U/ml

  • 種交差性

    交差種: Human

  • 製品の概要

    Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.


    ab108915 uPA Human Chromogenic Activity Assay Kit is developed to determine Human uPA activity in plasma and cell culture supernatants. The assay measures the ability of uPA to activate the plasminogen to plasmin in coupled or indirect assays that contain uPA, plasminogen, and a plasmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA in the reaction solution at 405 nm is directly proportional to the uPA enzymatic activity.


     


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • 試験プラットフォーム

    Microplate reader

製品の特性

画像

  • Indirect ELISA - Urokinase type plasminogen activator Human Chromogenic Activity Assay Kit (ab108915)
    Indirect ELISA - Urokinase type plasminogen activator Human Chromogenic Activity Assay Kit (ab108915)

    uPA measured in biologicals showing quantity (IU) per mL of sample tested.

  • Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)
    Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)

    uPA Chromogenic Activity Standard Curve (For High uPA Activity Samples)

  • Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)
    Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)

    uPA Chromogenic Activity Standard Curve (For Low uPA Activity Samples)

参考文献 (2)

ab108915 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108915 は 2 報の論文で使用されています。

  • Hohensinner PJ  et al. PAI-1 (Plasminogen Activator Inhibitor-1) Expression Renders Alternatively Activated Human Macrophages Proteolytically Quiescent. Arterioscler Thromb Vasc Biol N/A:N/A (2017). PubMed: 28818858
  • Gupta V  et al. Glaucoma is associated with plasmin proteolytic activation mediated through oxidative inactivation of neuroserpin. Sci Rep 7:8412 (2017). PubMed: 28827627

レビューと Q&A

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Question

I have one other question regarding the specificity of this assay. Since tissue associated plasminogen activator (tPA) can also convert plasminogen to plasmin, how can I be sure that I am measuring only uPA-mediated conversion of plasminogen to plasmin and not tPA-mediated conversion? It seems to me that what I will be measuring is the overall conversion of plasminogen to plasmin and not a uPA-specific conversion. Can you clarify this?

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Answer



For uPA activity kits, we have two types of kit. One is ab108916 which is direct using uPA substrate to generate the color. The uPA substrate is specifically designed for its specificity for uPA. This assay can differentiate uPA activity vs. tPA activity.

On the other hand, we also have an indirect uPA activity kit which is ab108915. This kit is using a plasmin substrate to measure uPA activity.

ab108916 can differentiate uPA vs. tPA activity, however, it is not as sensitive as ab108915 which is an indirect assay. ab108915 is more sensitive assay but is no able to differentiate uPA vs. tPA activity. We provide uPA standard for indirect assay is for reference of uPA activity.

If a cell culture sample specific for uPA expression is used, ab108915 will works well. However, if a plasma or serum sample is used, it is difficult to differentiate from tPA activity. But as the uPA activity accounts for at least 50 % of activity for cleave plasminogen to plasmin, the kit can at least provide a reference for uPA activity when samples are compared although we would recommend ab108916 if the samples have enough uPA.

Click here (or use the following: https://www.abcam.com/Urokinase-type-Plasminogen-Activator-Human-Chromogenic-Activity-Assay-Kit-ab108915.html).

Click here (or use the following: https://www.abcam.com/Urokinase-type-Plasminogen-Activator-Human-Chromogenic-Activity-Assay-Kit-Direct-Assay-ab108916.html).

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Question

Dear,

I'm interested in the ab108915 assay kit. However, I need more information on the kit contents to be able to decide if it is suited for my application. More specifically it is important to know what the pH is of the assay diluent. I can't find this information in the protocol or the SDS. It would be great if you can let me know.

Kind regards,

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Answer

Thank you for your enquiry.

I can confirm the pH for the assay diluent buffer is 8,5.

I hope this will be helpful. If you require any further details or information, please donot hesitate to let me know.

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