Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4809] to Urm1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
製品名Anti-Urm1 antibody [EPR4809] - BSA and Azide free
Urm1 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR4809] to Urm1 - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC,IHC-P or IP
Synthetic peptide within Human Urm1 aa 1-100. The exact sequence is proprietary.
ab247932 is the carrier-free version of ab110699 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247932 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.|
機能Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. May also act as an ubiquitin-like protein that is covalently conjugated to other proteins; the relevance of such function is however unclear in vivo.
パスウェイtRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
配列類似性Belongs to the URM1 family.
翻訳後修飾C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
- Information by UniProt
- 2900073H19Rik antibody
- C9orf74 antibody
- Chromosome 9 open reading frame 74 antibody
All lanes : Anti-Urm1 antibody [EPR4809] (ab110699) at 1/1000 dilution
Lane 1 : Human fetal liver lysate at 10 µg
Lane 2 : A549 lysate at 10 µg
Lane 3 : Human fetal lung lysate at 10 µg
Lane 4 : HepG2 lysate at 10 µg
Lane 5 : DDDDK-tagged Urm1 recombinant protein at 0.01 µg
Predicted band size: 11 kDa
This data was developed using ab110699, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.