Key features and details
- Rabbit polyclonal to Urm1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Urm1 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Urm1
アプリケーション適用あり: WBmore details
種交差性交差種: Saccharomyces cerevisiae
Recombinant full length protein (S. cerevisiae).
- Most yeast cell lysates can be used as a positive control without induction or stimulation.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
特記事項（精製）Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
Our Abpromise guarantee covers the use of ab4755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 12 kDa.|
機能Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. May also act as an ubiquitin-like protein that is covalently conjugated to other proteins; the relevance of such function is however unclear in vivo.
パスウェイtRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
配列類似性Belongs to the URM1 family.
翻訳後修飾C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
- Information by UniProt
- SwissProt: P40554 Saccharomyces cerevisiae
- 2900073H19Rik antibody
- C9orf74 antibody
- Chromosome 9 open reading frame 74 antibody
ab4755 was tested by immunoblot against yeast lysates expressing the Urm1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1/1000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1/15000 dilution of Donkey polyclonal to Goat IgG (HRP)(ab7125). Panel C shows specific reaction with Urm1 using a 1/1000 dilution of ab4755 followed by reaction with a 1/15000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by