Name: Anti-UQCRC2 antibody [13G12AF12BB11]
SKU: ab14745
Rating: 4 (11 reviews)

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Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

Key features and details

Mouse monoclonal [13G12AF12BB11] to UQCRC2
Suitable for: Flow Cyt, IHC-P, WB
Reacts with: Human
Isotype: IgG1

Alexa Fluor® 488 Alexa Fluor® 647 HRP

製品名

Anti-UQCRC2 antibody [13G12AF12BB11]
UQCRC2 一次抗体製品一覧
製品の詳細

Mouse monoclonal [13G12AF12BB11] to UQCRC2
由来種

Mouse
アプリケーション

適用あり: Flow Cyt, IHC-P, WBmore details
種交差性

交差種: Human
交差が予測される動物種: Pig
免疫原

Tissue, cells or virus corresponding to Cow UQCRC2.
ポジティブ･コントロール
- Human, bovine, murine and rat heart mitochondria. HepG2 cell lysate.
特記事項

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C. Do Not Freeze.
バッファー

pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
精製度

IgG fraction
ポリ/モノ

モノクローナル
クローン名

13G12AF12BB11
アイソタイプ

IgG1
軽鎖の種類

kappa
研究分野

Alternative Versions
Compatible Secondaries
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
- Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
Recombinant Protein
- Recombinant Human UQCRC2 protein (denatured) (ab134532)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab14745の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt		Use 1µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IF		Use a concentration of 0.1 - 0.5 µg/ml. Requires heat-induced antigen retrieval where aldehydes are used as fixatives.
IHC-P		Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB	(9)	Use a concentration of 1 µg/ml. Detects a band of approximately 49.5 kDa.

特記事項
Flow Cyt Use 1µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IF Use a concentration of 0.1 - 0.5 µg/ml. Requires heat-induced antigen retrieval where aldehydes are used as fixatives.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49.5 kDa.

機能

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
配列類似性

Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
細胞内局在

Mitochondrion inner membrane.
Target information above from: UniProt accession P22695 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 7385 Human
- Omim: 191329 Human
- SwissProt: P22695 Human
- Unigene: 528803 Human
別名
- Complex III subunit 2 antibody
- Core protein II antibody
- Cytochrome b c1 complex subunit 2 mitochondrial antibody
- Cytochrome b-c1 complex subunit 2 antibody
- EC 1.10.2.2 antibody
- MC3DN5 antibody
- mitochondrial antibody
- QCR2 antibody
- QCR2_HUMAN antibody
- Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody
- Ubiquinol cytochrome c reductase core protein II antibody
- Ubiquinol-cytochrome-c reductase complex core protein 2 antibody
- UQCR2 antibody
- Uqcrc2 antibody
see all

Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)This image is courtesy of an anonymous Abreview

Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745) at 1/1000 dilution + H23 whole cell lysate at 20 µg

Secondary
HRP-conjugated goat anti-mouse IgG polyclonal at 1/2000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Observed band size: 48 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

IHC image of ab14745 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14745, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Flow Cytometry - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

Overlay histogram showing HepG2 cells stained with ab14745 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14745, 1µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Immunofluorescence - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

Mitochondrial localization of complex III visualized by immunofluorescence using anti-complex III subunit Core 2 mAb 2E3GC12FB2AE2 (ab14745). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab14745 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

All lanes : Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745) at 0.5 µg/ml

Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to mouse IgG - H&L - pre-adsorbed (HRP) at 1/3000 dilution

Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.

Mouse on Mouse staining protocol

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab14745 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14745 は 233 報の論文で使用されています。

Todkar K et al. Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs. Nat Commun 12:1971 (2021). PubMed: 33785738
Acoba MG et al. The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism. Cell Rep 34:108869 (2021). PubMed: 33730581
Chen C et al. Imaging mass cytometry reveals generalised deficiency in OXPHOS complexes in Parkinson's disease. NPJ Parkinsons Dis 7:39 (2021). PubMed: 33980828
Oláhová M et al. POLRMT mutations impair mitochondrial transcription causing neurological disease. Nat Commun 12:1135 (2021). PubMed: 33602924
Sato M et al. The lncRNA Caren antagonizes heart failure by inactivating DNA damage response and activating mitochondrial biogenesis. Nat Commun 12:2529 (2021). PubMed: 33953175

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 18 Abreviews or Q&A

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Germline stem cells)

Gel Running Conditions

Reduced Denaturing (4-20%)

Loading amount

20 µg

Specification

Germline stem cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Jul 26 2019

Application

Western blot

Sample

Rat Tissue lysate - whole (Placenta)

Gel Running Conditions

Reduced Denaturing (10%)

Loading amount

20 µg

Specification

Placenta

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Jeremiah Gaudet

Verified customer

投稿 Mar 20 2018

Application

Western blot

Sample

American Robin (Turdis migratorius) Cell lysate - other (fibroblasts, mitochondrial fraction)

Gel Running Conditions

Reduced Denaturing (18%)

Loading amount

25 µg

Specification

fibroblasts, mitochondrial fraction

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Sep 12 2017

Application

Western blot

Sample

Mouse Cell lysate - other (AML12 hepatocytes, mitochondrial fraction)

Gel Running Conditions

Reduced Denaturing (18)

Loading amount

25 µg

Specification

AML12 hepatocytes, mitochondrial fraction

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Aug 22 2017

Application

Western blot

Sample

Drosophila melanogaster Tissue lysate - other (whole fly mitochondrial fraction)

Gel Running Conditions

Reduced Denaturing (18)

Loading amount

25 µg

Specification

whole fly mitochondrial fraction

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Aug 21 2017

Application

Western blot

Loading amount

30 µg

Gel Running Conditions

Reduced Denaturing (10% GEL)

Sample

Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)

Specification

Mouse Embryonic Fibroblasts

Blocking step

Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Abcam user community

Verified customer

投稿 May 22 2014

Application

Immunocytochemistry

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Sample

Human Cultured Cells (H460)

Specification

H460

Permeabilization

Yes - 0.5% Triton-X

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 May 20 2014

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (10% GEL)

Sample

Human Cell lysate - whole cell (H23)

Specification

H23

Blocking step

Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Feb 17 2014

Question

I need the MSDS for the following products ordered today with order number:

Anti-GRIM19 antibody [6E1BH7] (ab110240)
Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)
Anti-SDHB antibody [21A11AE7] (ab14714)
Anti-SDHA antibody [2E3GC12FB2AE2] - Mitochondrial Marker (ab14715)
Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)
Anti-FH antibody [8F12BB5] (ab110286)
Anti-Pyruvate Dehydrogenase E2 antibody [15D3G9C11 ] (ab110332)

Abcam community

Verified customer

Asked on Nov 23 2012

Answer

Thank you for contacting us.

The MSDS for these products have been added to each product datasheet. They should appear on the website within the next few hours (time for the cache to be regenerated).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Abcam Scientific Support

Answered on Nov 23 2012

Question

Dear Tech support

Are antibodies tested for WB expected to work with BN-PAGE method and is it guaranteed?

More specificaly I'm asking about the following antibodies:

ab14713
ab55521
ab110244
ab14715
ab14745
ab14705

As always, Thank you for your kind suppot.
Reagrds,

Abcam community

Verified customer

Asked on Aug 08 2012

Answer

Thank you for your enquiry.

I can confirm that the following antibodies are produced in our Mitosciences laboratories:

ab14713
ab110244
ab14715
ab14745
ab14705

These have been tested in blue native gel. These antibodies are also frequently referenced in the literature for Blue Native. An example is a paper attached showing these in BNPAGE.

The other antibody, ab55521 has not been tested in blue native gel. We do produce many products in house, but also source many externally. Therefore all our antibodieswill have been tested in different ways. Therefore, if an antibody has been tested in western blot, this does not mean it has also been tested in blue native gel. In this case, if you have the same question regarding any of our other antibodies, please do not hesitate to contact us again and we will be happy to confirm the information for you.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to let me know.

Abcam Scientific Support

Answered on Aug 08 2012

1-10 of 18 Abreviews or Q&A

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

軽鎖の種類

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (233)

レビューと Q&A

Western blot abreview for Anti-UQCRC2 antibody [13G12AF12BB11]

Western blot abreview for Anti-UQCRC2 antibody [13G12AF12BB11]

Western blot abreview for Anti-UQCRC2 antibody [13G12AF12BB11]

Western blot abreview for Anti-UQCRC2 antibody [13G12AF12BB11]

Western blot abreview for Anti-UQCRC2 antibody [13G12AF12BB11]

Western blot abreview for Anti-UQCRC2 antibody [13G12AF12BB11]

Immunocytochemistry abreview for Anti-UQCRC2 antibody [13G12AF12BB11]

Western blot abreview for Anti-UQCRC2 antibody [13G12AF12BB11]