Anti-uPA Receptor/U-PAR 抗体 (ab3129)

Submit a review Q&A (3)References (3)

Key features and details

  • Goat polyclonal to uPA Receptor/U-PAR
  • Suitable for: IP, Neutralising
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-uPA Receptor/U-PAR antibody
    uPA Receptor/U-PAR 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to uPA Receptor/U-PAR

  • 由来種

    Goat

  • アプリケーション

    適用あり: IP, Neutralisingmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein corresponding to Human uPA Receptor/U-PAR.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 5 - 10 µg/ml.
Neutralising Use a concentration of 2 - 4 µg/ml.

ターゲット情報

  • 機能

    Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

  • 組織特異性

    Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.

  • 配列類似性

    Contains 3 UPAR/Ly6 domains.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 87 antibody
      • CD87 antibody
      • CD87 antigen antibody
      • MO 3 antibody
      • MO3 antibody
      • Monocyte activation antigen Mo3 antibody
      • Plasminogen activator receptor urokinase antibody
      • Plasminogen activator urokinase receptor antibody
      • PLAUR antibody
      • U PAR antibody
      • u plasminogen activator receptor antibody
      • U-PAR antibody
      • u-plasminogen activator receptor form 2 antibody
      • UPA receptor antibody
      • uPAR antibody
      • UPAR_HUMAN antibody
      • Urinary plasminogen activator receptor antibody
      • URKR antibody
      • Urokinase plasminogen activator receptor antibody
      • Urokinase plasminogen activator surface receptor antibody
      • urokinase-type plasminogen activator (uPA) receptor antibody
      see all

    参考文献 (3)

    ab3129 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3129 は 3 報の論文で使用されています。

    • Pavet V  et al. Plasminogen activator urokinase expression reveals TRAIL responsiveness and supports fractional survival of cancer cells. Cell Death Dis 5:e1043 (2014). WB . PubMed: 24481457
    • Al-Souhibani N  et al. The RNA-binding zinc-finger protein tristetraprolin regulates AU-rich mRNAs involved in breast cancer-related processes. Oncogene : (2010). WB ; Human . PubMed: 20498646
    • Bezuidenhout L  et al. Ang-2 and PDGF-BB cooperatively stimulate human peripheral blood monocyte fibrinolysis. J Leukoc Biol 81:1496-503 (2007). Neutralising ; Human . PubMed: 17395886

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Question

    Thank you for kindly reply. I measured annexin v and Propidium Iodide(PI) in FACS, about 90 % cells were PI staining positive. So I considered cells were necrosis, moreover under the microscope cells are detached and morphological change occur and no fungus/bacteria contamination were obvious. According to your advice we use 0.5 ug/ml  concentration because when I used this concentration %PI positive cells was normal distribution. And I could not find answer of the last question about this product. Are there no changes in this product from 2007 to present? Sincerely yours  

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    Answer

    Thank you for your email. The lot number customer used in 2007 was different than you have been using. So we would expect some variations due to cell line or other lab related factors. I can assure you that there is no change in the antibody itself other than difference in lot. We are also not sure whether this antibody has any lot related issues, so I am sorry I am unable to comment on this. I would also like to ask is this antibody able to neutralize the effect of uPA? I hope this information will help. Should you have any other question please do not hesitate to ask.

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    Question

    Dear Sir/Madam I purchased your product  "uPA receptor antibody cat.no, ab3129". I used this antibody in order to neutralize uPA receptor, so I added this antibody into culture medium for 24 hrs. I cultured human pulmonary artery smooth muscle cells (invitrogen) in 0.5 % fcs/Medium231(invitrogen). Then I measured apoptosis using annexin v and staining method by FACS. Surprisingly when I added 2-4ug/ml concentration of this antibody, cells were almost died. So, I have a question. Is this antibody have cell toxicity component? According to reference that was mentioned in datasheet "Bezuidenhout L et al. Ang-2 and PDGF-BB cooperatively stimulate human peripheral blood monocyte fibrinolysis. J Leukoc Biol 81:1496-503 (2007)" , cells was not died. But this paper was published 2007 ,are there any changes in this product from 2007 to present? And when I use 0.5ug/ml concentration of this product, cells were alive. Can I use this concentration as blocking uPA receptor?                

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    Answer

    Thank you for contacting us. Could you specify are the cells just dying or there is some kind of fungus or bacterial growth? The antibody solution only contain sodium azide as cytotoxic compound however the affect of this would be minimal at the Abcam recommended dilutions. The recommended concentration and dilutions to use is between 2-4 ug/ml. However this is for guideline purpose only. The dilutions have to be determined by end user because the conditions vary from cells to cells. We would recommend using a range of dilution e.g. 0.5 ug/ml, 1 ug/ml and 2 ug/ml in order to get optimized neutralization in the cell line you have been using. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Could you help recommend a standard/positive control for uPAR run on an SDS-PAGE gel? Our customer is running homogenized mouse skeletal muscle. Thanks!

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    Answer

    Thank you for your enquiry. Ab3129 has not yet been tested for cross-reactivity with mouse uPA, so I would suggest a control from human samples. For example, uPA and uPAR are known to be overexpressed in mesenchymal and epithelial tumor cells. If you have any additional questions, please contact us again.

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