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  1. Link

    ugt1a6-antibody-ab97646.pdf

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Signal Transduction Metabolism Drug metabolism
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Anti-UGT1A6 抗体 (ab97646)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (1)

Product price, shipping and contact information

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Western blot - Anti-UGT1A6 antibody (ab97646)

    Key features and details

    • Rabbit polyclonal to UGT1A6
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-UGT1A6 antibody [EPR11068] (ab157476)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産
    医薬用外劇物

    製品の概要

    • 製品名

      Anti-UGT1A6 antibody
      UGT1A6 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to UGT1A6
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Human
    • 免疫原

      Amino acids 8-197 of Human UGT1A6 (AAH19861).

    • ポジティブ・コントロール

      • 293T, HeLa, HepG2, MOLT4 and Raji cell lines. A431 and H1299 whole cell lysates.
    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Signal Transduction
      • Metabolism
      • Drug metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Drug metabolism
      • Metabolism
      • Types of disease
      • Cancer

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa membrane extract lysate (ab29547)
      • Raji membrane extract lysate (ab30126)
      • A-431 whole cell lysate (ab7909)
    • Recombinant Protein

      • Recombinant Human UGT1A6 protein (ab162788)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab97646の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (2)
    Use at an assay dependent dilution. Predicted molecular weight: 61 kDa.
    特記事項
    WB
    Use at an assay dependent dilution. Predicted molecular weight: 61 kDa.

    ターゲット情報

    • 機能

      UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform has specificity for phenols.
    • 組織特異性

      Expressed in skin, kidney and liver.
    • 配列類似性

      Belongs to the UDP-glycosyltransferase family.
    • 細胞内局在

      Microsome. Endoplasmic reticulum membrane.
    • Target information above from: UniProt accession P19224 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 54578 Human
      • Omim: 606431 Human
      • SwissProt: P19224 Human
      • Unigene: 554822 Human
      • 別名

        • GNT1 antibody
        • HLUGP antibody
        • HLUGP1 antibody
        • Phenol metabolizing UDP glucuronosyltransferase antibody
        • Phenol-metabolizing UDP-glucuronosyltransferase antibody
        • UD16_HUMAN antibody
        • UDP glucuronosyltransferase 1 family polypeptide A6 antibody
        • UDP glucuronosyltransferase 1A6 antibody
        • UDP glycosyltransferase 1 family polypeptide A6 antibody
        • UDP-glucuronosyltransferase 1-6 antibody
        • UDP-glucuronosyltransferase 1-F antibody
        • UDP-glucuronosyltransferase 1A6 antibody
        • UDPGT antibody
        • UDPGT 1-6 antibody
        • UGT 1F antibody
        • UGT-1F antibody
        • UGT1 antibody
        • UGT1 06 antibody
        • UGT1*6 antibody
        • UGT1-06 antibody
        • UGT1.6 antibody
        • UGT1A6 antibody
        • UGT1A6S antibody
        • UGT1F antibody
        see all

      画像

      • Western blot - Anti-UGT1A6 antibody (ab97646)
        Western blot - Anti-UGT1A6 antibody (ab97646)
        All lanes : Anti-UGT1A6 antibody (ab97646) at 1/1000 dilution

        Lane 1 : A431 whole cell lysate
        Lane 2 : H1299 whole cell lysate

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 61 kDa



        7.5% SDS PAGE

      プロトコール

      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (1)

      ab97646 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab97646 は 1 報の論文で使用されています。

      • Wen X  et al. Regulation of hepatic phase II metabolism in pregnant mice. J Pharmacol Exp Ther 344:244-52 (2013). WB ; Mouse . PubMed: 23055538

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-UGT1A6 antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - other (mouse hepatoma cell line)
      Gel Running Conditions
      Reduced Denaturing (10% gel)
      Loading amount
      10 µg
      Specification
      mouse hepatoma cell line
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Mar 15 2021

      Western blot abreview for Anti-UGT1A6 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Liver)
      Loading amount
      50 µg
      Specification
      Liver
      Gel Running Conditions
      Reduced Denaturing (NuPAGE Novex 4-12% Bis-Tris mini gel from Invitrogen)
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Xia Wen

      Verified customer

      投稿 Jul 25 2011

      Question

      WB, RIPA, sample buffer boil 5 min, 50 ug protein, ponceau shows protein, actin works well, other targets work well, sample lysates working well for others in lab. block 1 hr 5% milk

      Read More

      Abcam community

      Verified customer

      Asked on Nov 29 2012

      Answer

      Thank you for contacting us. Your credit note ID is xxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

      Read More

      Abcam Scientific Support

      Answered on Nov 29 2012

      Question

      Hi Technical,

      Kindly advise ifAB97646 ( Anti-UGT1A6 antibody )& AB72598 (Anti-Cytochrome P450 2C8 + 2C9 + 2C18 + 2C19 antibody)are suitable for use with Rat samples. Application is Western Blot.

      Thank you and regards

      Read More

      Abcam community

      Verified customer

      Asked on Jul 10 2012

      Answer

      Thank you for your enquiry.

      I am sorry to confirm that as far as we are aware, ab72598 and ab97646 Cytochrome P450 2C8 + 2C9 + 2C18 + 2C19 antibodies have never been tested with samples from rat. All tested species cross-reactivitycovered by our guaranteeis stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

      I have checked the alignment of the rat protein with the immunogen sequences for the antibodies:

      ab72598 immunogen ##########
      16% alignment to the rat

      ab97646 immungoen from a region within amino acids ####
      73% alignment to the rat

      We recommend that alignment should be over 85% to predict that an antibody will detect in a different species. However, we cannot guarantee this without first testing. As ab97646 has the higher alignment, this would be the preferable antibody to try. At 73% alignment, there may still be some crossreactivity. We would not suggest to try ab72598 in rat as the alignment is so low.

      If you would like to test ab97646 in rat, please contact me again by replying to this message prior to the purchase as you may be eligible for our testing discount program.

      Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

      To find out more about our Abreview system, please see the following link:

      https://www.abcam.com/abreviews

      I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

      Read More

      Abcam Scientific Support

      Answered on Jul 10 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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