製品の概要
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製品名Anti-UCP2 antibody
UCP2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to UCP2
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由来種Rabbit
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アプリケーション適用あり: IHC-P, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Hamster, Cat, Dog, Pig, Orangutan
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human UCP2.
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ポジティブ･コントロール
- This antibody gave a positive signal in Human Adipose and Spleen Tissue Lysates and FFPE human lymph node tissue sections.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab97931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa). |
ターゲット情報
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機能UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
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組織特異性Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle.
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配列類似性Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
細胞内局在Mitochondrion inner membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 7351 Human
- Entrez Gene: 22228 Mouse
- Entrez Gene: 397549 Pig
- Entrez Gene: 54315 Rat
- Omim: 601693 Human
- SwissProt: P55851 Human
- SwissProt: P70406 Mouse
- SwissProt: O97562 Pig
see all -
別名
- BMIQ4 antibody
- Mitochondrial uncoupling protein 2 antibody
- SLC25A8 antibody
see all
画像
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Western blot - Anti-UCP2 antibody (ab97931)All lanes : Anti-UCP2 antibody (ab97931) at 1 µg/ml
Lane 1 : Human adipose normal tissue lysate - total protein (ab28980)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 30 kDa (why is the actual band size different from the predicted?)
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP2 antibody (ab97931)IHC image of ab97931 staining in human lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97931, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
参考文献
This product has been referenced in:
- Koziel A & Jarmuszkiewicz W Hypoxia and aerobic metabolism adaptations of human endothelial cells. Pflugers Arch 469:815-827 (2017). WB . Read more (PubMed: 28176017) »
- Eirin A et al. Restoration of Mitochondrial Cardiolipin Attenuates Cardiac Damage in Swine Renovascular Hypertension. J Am Heart Assoc 5:N/A (2016). Read more (PubMed: 27247333) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"