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  1. Link

    ucp2-antibody-ab97931.pdf

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Cancer Cancer Metabolism Metabolic signaling pathway Integration of energy metabolism
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Anti-UCP2 抗体 (ab97931)

  • Datasheet
Reviews (5)Q&A (2)References (21)

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Abpromise

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Western blot - Anti-UCP2 antibody (ab97931)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP2 antibody (ab97931)

Key features and details

  • Rabbit polyclonal to UCP2
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

こちらの製品もご検討ください

一次抗体
Product image
Anti-UCP3 antibody (ab10985)
一次抗体
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Anti-Citrate synthetase antibody [EPR8067] (ab129095)
タンパク質
Product image
Recombinant Human UCP2 protein (ab152783)

関連製品

製品の概要

  • 製品名

    Anti-UCP2 antibody
    UCP2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to UCP2
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-P, WBmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Hamster, Cat, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human UCP2 aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107707)

  • ポジティブ・コントロール

    • This antibody gave a positive signal in Human Adipose and Spleen Tissue Lysates and FFPE human lymph node tissue sections.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human UCP2 protein (ab152783)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab97931の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (4)
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa).
特記事項
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa).

ターゲット情報

  • 機能

    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • 組織特異性

    Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle.
  • 配列類似性

    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • 細胞内局在

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession P55851 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 7351 Human
    • Entrez Gene: 22228 Mouse
    • Entrez Gene: 397549 Pig
    • Entrez Gene: 54315 Rat
    • Omim: 601693 Human
    • SwissProt: P55851 Human
    • SwissProt: P70406 Mouse
    • SwissProt: O97562 Pig
    • SwissProt: P56500 Rat
    • Unigene: 80658 Human
    • Unigene: 171378 Mouse
    • Unigene: 13333 Rat
    see all
  • 別名

    • BMIQ4 antibody
    • Mitochondrial uncoupling protein 2 antibody
    • SLC25A8 antibody
    • Solute carrier family 25 member 8 antibody
    • UCP 2 antibody
    • ucp2 antibody
    • UCP2_HUMAN antibody
    • UCPH antibody
    • Uncoupling protein 2 antibody
    • Uncoupling protein 2 mitochondrial proton carrier antibody
    see all

画像

  • Western blot - Anti-UCP2 antibody (ab97931)
    Western blot - Anti-UCP2 antibody (ab97931)
    All lanes : Anti-UCP2 antibody (ab97931) at 1 µg/ml

    Lane 1 : Human adipose normal tissue lysate - total protein (ab28980)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP2 antibody (ab97931)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP2 antibody (ab97931)
    IHC image of ab97931 staining in human lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97931, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

プロトコール

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (21)

ab97931 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab97931 は 21 報の論文で使用されています。

  • Mao JY  et al. The effects of UCP2 on autophagy through the AMPK signaling pathway in septic cardiomyopathy and the underlying mechanism. Ann Transl Med 9:259 (2021). PubMed: 33708886
  • Yang Y  et al. Berberine induces lipolysis in porcine adipocytes by activating the AMP-activated protein kinase pathway. Mol Med Rep 21:2603-2614 (2020). PubMed: 32323785
  • Broniarek I  et al. The Influence of Statins on the Aerobic Metabolism of Endothelial Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32098258
  • Qiu H  et al. Regulation and Mechanism of miR-518d through the PPARa-Mediated NF-?B Pathway in the Development of Gestational Diabetes Mellitus. J Diabetes Res 2020:7019597 (2020). PubMed: 33123597
  • Wei W  et al. Rosuvastatin Alleviates Ischemia/Reperfusion Injury in Cardiomyocytes by Downregulating Hsa-miR-24-3p to Target Upregulated Uncoupling Protein 2. Cell Reprogram 21:99-107 (2019). PubMed: 30835496
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-UCP2 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (kidney cortex, heart, liver, spleen)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
30 µg
Specification
kidney cortex, heart, liver, spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 22 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-UCP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (DU145 prostate cancer cell line)
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4, 5 min at RT
Specification
DU145 prostate cancer cell line
Blocking step
(agent) for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

投稿 Nov 20 2017

Western blot abreview for Anti-UCP2 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Sample
Human Cell lysate - other (Heterologously expressed in Lactococcus lactis)
Specification
Heterologously expressed in Lactococcus lactis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Martin King

Verified customer

投稿 Jul 31 2014

Western blot abreview for Anti-UCP2 antibody

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
mouse embryonic fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 29 2014

Western blot abreview for Anti-UCP2 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human endotherial cells)
Loading amount
15 µg
Specification
Human endotherial cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 16 2011

Question

Hei,
I am looking for anti-mouse UCP2 antibody for EM application. But I
like to get reference paper/articles before buying. I got data sheet
but i dont see any reference paper for it. Can you please send me any
reference paper for it.
https://www.abcam.com/UCP2-antibody-ab97931.pdf
Best Regrads,

Read More

Abcam community

Verified customer

Asked on Sep 26 2012

Answer

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab97931 has never been tested in EM. All tested applications covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

I can also confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in many cases, regrettably we do not have access to all the articles mentioning our products, or subscriptions to all the journals that publish them. I'm sorry there are none to provide on this occasion for ab97931.

I can also provide you with a 5% discount if you wish to purchase this antibody to try in EM. In order to obtain this discount, please use the following code when you place the order:

########


Also, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More

Abcam Scientific Support

Answered on Sep 26 2012

Question

Inquiry: I want to detect uncoupling protein 2 (UCP2) in paraformaldehyde-fixed primate tissue by immunohistochemistry. Are any of your Abs suitable for this? Many thanks

Read More

Abcam community

Verified customer

Asked on May 18 2012

Answer

Thank you for contacting us.

We currently offer one product which has been tested in IHC-P and is predicted to work in primates. Ab97931 (https://www.abcam.com/UCP2-antibody-ab97931.html).

Though predicted to work based upon sequence homology, this product has not been tested in primate.For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


I hope this information is helpful to you. If you are interested in this offer please let me know. Also, please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on May 18 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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