Anti-UCP2 抗体 (ab97931)
Key features and details
- Rabbit polyclonal to UCP2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-UCP2 antibody
UCP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UCP2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Hamster, Cat, Dog, Pig, Orangutan
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免疫原
Synthetic peptide corresponding to Human UCP2 aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab107707) -
ポジティブ･コントロール
- This antibody gave a positive signal in Human Adipose and Spleen Tissue Lysates and FFPE human lymph node tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97931の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB | (4) |
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa).
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa). |
ターゲット情報
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機能
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. -
組織特異性
Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle. -
配列類似性
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7351 Human
- Entrez Gene: 22228 Mouse
- Entrez Gene: 397549 Pig
- Entrez Gene: 54315 Rat
- Omim: 601693 Human
- SwissProt: P55851 Human
- SwissProt: P70406 Mouse
- SwissProt: O97562 Pig
see all -
別名
- BMIQ4 antibody
- Mitochondrial uncoupling protein 2 antibody
- SLC25A8 antibody
see all
画像
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Western blot - Anti-UCP2 antibody (ab97931)All lanes : Anti-UCP2 antibody (ab97931) at 1 µg/ml
Lane 1 : Human adipose normal tissue lysate - total protein (ab28980)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP2 antibody (ab97931)IHC image of ab97931 staining in human lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97931, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (21)
ab97931 は 21 報の論文で使用されています。
- Mao JY et al. The effects of UCP2 on autophagy through the AMPK signaling pathway in septic cardiomyopathy and the underlying mechanism. Ann Transl Med 9:259 (2021). PubMed: 33708886
- Yang Y et al. Berberine induces lipolysis in porcine adipocytes by activating the AMP-activated protein kinase pathway. Mol Med Rep 21:2603-2614 (2020). PubMed: 32323785
- Broniarek I et al. The Influence of Statins on the Aerobic Metabolism of Endothelial Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32098258
- Qiu H et al. Regulation and Mechanism of miR-518d through the PPARa-Mediated NF-?B Pathway in the Development of Gestational Diabetes Mellitus. J Diabetes Res 2020:7019597 (2020). PubMed: 33123597
- Wei W et al. Rosuvastatin Alleviates Ischemia/Reperfusion Injury in Cardiomyocytes by Downregulating Hsa-miR-24-3p to Target Upregulated Uncoupling Protein 2. Cell Reprogram 21:99-107 (2019). PubMed: 30835496
