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  1. Link

    ucp1-antibody-ab23841.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-UCP1 抗体 (ab23841)

  • Datasheet
  • SDS
Reviews (9)Q&A (10)References (118)

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Western blot - Anti-UCP1 antibody (ab23841)
  • Immunohistochemistry - Anti-UCP1 antibody (ab23841)

Key features and details

  • Rabbit polyclonal to UCP1
  • Suitable for: WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-UCP1 antibody [EPR23004-34] (ab234430)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-UCP1 antibody
    UCP1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to UCP1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Mouse, Rat
    交差が予測される動物種: Hamster, Dog, Human, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to Human UCP1 aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab24282)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
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    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunizing Peptide (Blocking)

    • Human UCP1 peptide (ab24282)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human UCP1 protein (ab152782)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab23841の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (4)
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

ターゲット情報

  • 機能

    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • 組織特異性

    Brown adipose tissue.
  • 配列類似性

    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • 細胞内局在

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession P25874 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 7350 Human
    • Entrez Gene: 22227 Mouse
    • Entrez Gene: 24860 Rat
    • Omim: 113730 Human
    • SwissProt: P25874 Human
    • SwissProt: P12242 Mouse
    • SwissProt: P04633 Rat
    • Unigene: 249211 Human
    • Unigene: 4177 Mouse
    • Unigene: 10281 Rat
    see all
  • 製品の状態

    UCP1 is preferentially expressed in brown adipose tissue
  • 別名

    • mitochondrial brown fat uncoupling protein antibody
    • Mitochondrial brown fat uncoupling protein 1 antibody
    • SLC25A7 antibody
    • Solute carrier family 25 member 7 antibody
    • Thermogenin antibody
    • UCP 1 antibody
    • UCP antibody
    • UCP1 antibody
    • UCP1_HUMAN antibody
    • uncoupling protein 1 (mitochondrial, proton carrier) antibody
    • Uncoupling protein 1 antibody
    see all

画像

  • Western blot - Anti-UCP1 antibody (ab23841)
    Western blot - Anti-UCP1 antibody (ab23841)
    All lanes : Anti-UCP1 antibody (ab23841) at 1 µg/ml

    Lane 1 : Adult Mouse Brown Adipose Tissue Lysate
    Lane 2 : Adult Rat Brown Adipose Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa
    Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • Immunohistochemistry - Anti-UCP1 antibody (ab23841)
    Immunohistochemistry - Anti-UCP1 antibody (ab23841)Image from Liu W et al., PLoS Genet. 2006;8:11958-63. Fig 5.; doi: 10.1371/journal.pgen.1003626 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    ab23841 staining UCP1 in serial sections of white fat from mice by immunohistochemistry. Serial sections were de-paraffinized, rehydrated through xylene, ethanol and water; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 60 minutes. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody.

     

プロトコール

  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (118)

ab23841 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab23841 は 118 報の論文で使用されています。

  • Giori IG  et al. Enalapril and treadmill running reduce adiposity, but only the latter causes adipose tissue browning in mice. J Cell Physiol 236:900-910 (2021). PubMed: 32617979
  • Lee DH  et al. Withaferin A exerts an anti-obesity effect by increasing energy expenditure through thermogenic gene expression in high-fat diet-fed obese mice. Phytomedicine 82:153457 (2021). PubMed: 33444942
  • Pan XX  et al. Senescent T Cell Induces Brown Adipose Tissue "Whitening" Via Secreting IFN-?. Front Cell Dev Biol 9:637424 (2021). PubMed: 33748126
  • Whitehead A  et al. Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis. Nat Commun 12:1905 (2021). PubMed: 33772024
  • Voisin M  et al. Inhibiting LXRa phosphorylation in hematopoietic cells reduces inflammation and attenuates atherosclerosis and obesity in mice. Commun Biol 4:420 (2021). PubMed: 33772096
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 19 Abreviews or Q&A

Western blot abreview for Anti-UCP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brown fat)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Brown fat
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

投稿 Dec 03 2018

Immunohistochemistry (Frozen sections) abreview for Anti-UCP1 antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brown adipose tissue and aortic root)
Permeabilization
Yes - Triton X-100 (0,1% in PBS)
Specification
brown adipose tissue and aortic root
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Aug 04 2017

Western blot abreview for Anti-UCP1 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human pre-adipocytes overexpressing hUCP1)
Gel Running Conditions
Non-reduced Denaturing (4-15%)
Loading amount
30 µg
Specification
Human pre-adipocytes overexpressing hUCP1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Read More

Morten Lundh

Verified customer

投稿 Jul 18 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-UCP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (A549 Lung cance cells)
Specification
A549 Lung cance cells
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Read More

DR. Dimitra Kalamida

Verified customer

投稿 Feb 20 2015

Western blot abreview for Anti-UCP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (10.5%)
Sample
Mouse Tissue lysate - other (brown fat)
Specification
brown fat
Treatment
cold stress
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Read More

DR. Kun Dong

Verified customer

投稿 Jul 09 2013

Question

I’m very interested in purchasing anti-UCP1 antibody (ab23841) with custom fluorescence conjugate like Alexa Fluor 647. I want to know how long it will take to get fluorescence-conjugated anti-UCP1 antibody. I also want to know how to place the order like this and how much it will cost.

Read More

Abcam community

Verified customer

Asked on Feb 28 2012

Answer

Thank you for your inquiry.


We do not currently offer this antibody directly conjugated to a fluorophore.I could see if the lab would be able to generate this for you, but we do not carry Alexa Fluors. We may be able to provide this service if you order in bulk (>10 units) with a Cy5 conjugation. If you would be interested in this, please email my colleage Andrea who handles bulk requests at mailto:us.sales@abcam.com.


Alternatively, you could do the labeling yourself using one of our 3-step EasyLink conjugation kits. Again, we don't carry Alexa Fluors, but you would be able to label Cy5.


https://www.abcam.com/index.html?pageconfig=resource&rid=13148


In order to use the conjugation kit, like ab102879 ($335.00), with UCP1 ab23841, you would first need to remove some of the BSA in the antibody as this can interfere with the labeling reaction. See our FAQs:


https://www.abcam.com/index.html?pageconfig=resource&rid=13156#4


https://www.abcam.com/EasyLink-Cy5-5-Conjugation-Kit-3-x-100%C2%B5g-Cy5-5-ab102879.html


You can remove the BSA using a purification kit like ab102783 (1 purification, $221.00) or ab102784 (3 purifications, $438.00).


https://www.abcam.com/Antibody-Purification-kit-1-purification-ab102783.html


https://www.abcam.com/Antibody-Purification-kit-3-purifications-ab102784.html


I hope this information helps. Please contact us with any other questions.

Read More

Abcam Scientific Support

Answered on Feb 28 2012

Question

Have you done a blocking experiment with the antibody and peptide? Do you have an image?

Read More

Abcam community

Verified customer

Asked on Nov 29 2012

Answer

Thank you for contacting us.

I've attached one of our blocking experiments with the antibody and peptide combination.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Nov 29 2012

Question

Ab47431 gave a band around 50 kDa instead of 150 kDa, and ab23841 did not show any bands.

Read More

Abcam community

Verified customer

Asked on Aug 24 2012

Answer

Thank you very much for your call today and for letting us know about the trouble with these antibodies.

I'm sending a free of charge vial of ab14571 on the order *** which should arrive on Tuesday, to replace the vial of ab47431. Please keep me updated about the results with ab23841 after trying the alterations we discussed.

I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

Read More

Abcam Scientific Support

Answered on Aug 24 2012

Question

The most recent vial failed in western blotting at 1/1000 (no signal) compared to an earlier lot that worked well at 1/10,000.

Read More

Abcam community

Verified customer

Asked on Aug 14 2012

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on Aug 14 2012

Question

WB with mouse brown adipocytes
sees band at 37 kDa, but should be at 33 kDa
other Ab from another company detects right band size
treatment of cells should increase band intensity, but this is not seen either
30 ug protein loaded
Ab: 1/1000
secondary: OK

Read More

Abcam community

Verified customer

Asked on Apr 03 2012

Answer

Thank you for contacting us.

Your credit note ID is xxx.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Abcam Scientific Support

Answered on Apr 03 2012

1-10 of 19 Abreviews or Q&A

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