Application
Western blot
Sample
Sheep Cell lysate - whole cell (preadipocyte)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% PAG)
Loading amount
10 µg
Specification
preadipocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA/TBS-T
Dilution
1/750
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 32 kDa
Positive control
b-Actin loading control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Barbara Makela
Verified customer
投稿 Apr 15 2016