Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4896] to UCH37 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt, WB, IP
- Reacts with: Rat, Human
製品名Anti-UCH37 antibody [EPR4896] - BSA and Azide free
UCH37 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR4896] to UCH37 - BSA and Azide free
アプリケーション適用あり: IHC-P, Flow Cyt, WB, IPmore details
種交差性交差種: Rat, Human
Synthetic peptide within Human UCH37 aa 1-100. The exact sequence is proprietary.
Ab248062 is the carrier-free version of ab124931. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248062 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degree C, below boiling, and then let cool for 10-20 min.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).|
|IP||Use at an assay dependent concentration.|
機能Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.
配列類似性Belongs to the peptidase C12 family.
細胞内局在Cytoplasm. Nucleus. Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus.
- Information by UniProt
- UCH37 antibody
- AD-019 antibody
- CGI 70 antibody
All lanes : Anti-UCH37 antibody [EPR4896] (ab124931) at 1/1000 dilution
Lane 1 : Human fetal kidney cell lysates
Lane 2 : 293T cell lysates
Lane 3 : NCCIT cell lysates
Lane 4 : SH-SY5Y cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa
This data was developed using ab124931, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.