Anti-UCH37 抗体 [EP4897] (ab133508)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4897] to UCH37
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-UCH37 antibody [EP4897]
UCH37 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP4897] to UCH37 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human UCH37 aa 250-350. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Human colon, Mouse breast, and Rat liver lysates. IHC-P: Human colorectal carcinoma, Mouse liver, Mouse liver cancer, and Rat liver.
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特記事項
This product has switched from a hybridoma to recombinant production method on 25th May 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP4897 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133508の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/10000. Predicted molecular weight: 38 kDa.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/10000. Predicted molecular weight: 38 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. -
配列類似性
Belongs to the peptidase C12 family. -
細胞内局在
Cytoplasm. Nucleus. Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 51377 Human
- Entrez Gene: 56207 Mouse
- Entrez Gene: 360853 Rat
- Omim: 610667 Human
- SwissProt: Q9Y5K5 Human
- SwissProt: Q9WUP7 Mouse
- Unigene: 591458 Human
- Unigene: 261004 Mouse
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別名
- UCH37 antibody
- AD-019 antibody
- CGI 70 antibody
see all
画像
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All lanes : Anti-UCH37 antibody [EP4897] (ab133508) at 1/10000 dilution
Lane 1 : Human colon lysate
Lane 2 : Mouse breast lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 38 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human colorectal carcinoma tissue labelling UCH37 with ab133508 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human colorectal carcinoma. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling UCH37 with ab133508 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Mouse liver. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded Mouse liver cancer tissue labelling UCH37 with ab133508 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Mouse liver cancer. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling UCH37 with ab133508 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Rat liver. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab133508 は 13 報の論文で使用されています。
- Deng Y et al. ARV-771 Acts as an Inducer of Cell Cycle Arrest and Apoptosis to Suppress Hepatocellular Carcinoma Progression. Front Pharmacol 13:858901 (2022). PubMed: 35600879
- Song A et al. Branched ubiquitin chain binding and deubiquitination by UCH37 facilitate proteasome clearance of stress-induced inclusions. Elife 10:N/A (2021). PubMed: 34761751
- Lu X et al. Structure-guided bifunctional molecules hit a DEUBAD-lacking hRpn13 species upregulated in multiple myeloma. Nat Commun 12:7318 (2021). PubMed: 34916494
- Guo S et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 12:42 (2021). PubMed: 33414446
- Dickson P et al. Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol 27:1371-1382.e6 (2020). PubMed: 32857985