Immunocytochemistry/ Immunofluorescence abreview for Anti-Ubiquitin (linkage-specific K48) antibody [EP8589]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
fish gelatine buffer with 0.1% casein and 5% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C
Sample
Mouse Cell (immortalised bone marrow-derived macrophages)
Specification
immortalised bone marrow-derived macrophages
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: blocking buffer
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Alexa-fluor 568 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 568
Dilution
1/1000

Dr. James Harris

Verified customer

投稿 Sep 08 2014

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