Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Mouse Cell lysate - whole cell (MEFs)
Specification
MEFs
Treatment
-/+ 4 h MG132 (25 μM)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/20000
Secondary antibody
Name
Non-Abcam antibody was used: Pierce 31210
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: Long ubiquitin chain kDa
Exposure
30 second(s)
Positive control
MG-132 treated MEF WCL
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 19 2013