Anti-Ubiquitin (linkage-specific K27) 抗体 [EPR17034] - BSA and Azide free (ab238442)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17034] to Ubiquitin (linkage-specific K27) - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra), IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
製品の概要
-
製品名
Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free
Ubiquitin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17034] to Ubiquitin (linkage-specific K27) - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra), IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human, Recombinant fragment -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- IHC-P: Human transitional cell carcimona of the bladder tissue.
-
特記事項
ab238442 is the carrier-free version of ab181537.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17034 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
- Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)
- Alexa Fluor® 488 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237254)
- Alexa Fluor® 647 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237255)
- HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237256)
-
Compatible Secondaries
-
Conjugation kits
-
Immunohistochemistry kits
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab238442の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
Actual Band: 14kDa and above. |
|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
特記事項 |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa. Actual Band: 14kDa and above. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
-
関連性
Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains. -
細胞内局在
Cell Membrane, Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 7314 Human
- Entrez Gene: 22187 Mouse
- Entrez Gene: 192255 Rat
- SwissProt: P0CG47 Human
- SwissProt: P0CG48 Human
- SwissProt: P62979 Human
- SwissProt: P62987 Human
- SwissProt: P62988 Human
see all -
別名
- Epididymis secretory protein Li 50 antibody
- FLJ25987 antibody
- HEL S 50 antibody
see all
画像
-
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma)
labelling Ubiquitin (linkage-specific K27) with purified ab181537 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181537).
-
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Mouse spleen is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181537).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Rat spleen is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181537).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/160 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) and cell without incubation with primary antibody and secondary antibody is blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181537).
-
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on cancer cells of Human bladder transitional cell carcinoma is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181537).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab238442 は論文での使用が確認できていません。