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    ubiquitin-antibody-ab7780.pdf

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Anti-Ubiquitin 抗体 (ab7780)

  • Datasheet
  • SDS
Reviews (33)Q&A (17)References (226)

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Immunocytochemistry - Anti-Ubiquitin antibody (ab7780)
  • Immunocytochemistry - Anti-Ubiquitin antibody (ab7780)
  • Immunocytochemistry - Anti-Ubiquitin antibody (ab7780)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody (ab7780)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody (ab7780)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ubiquitin antibody (ab7780)

Key features and details

  • Rabbit polyclonal to Ubiquitin
  • Suitable for: ICC, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Ubiquitin antibody [EPR8830] (ab134953)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Ubiquitin antibody
    Ubiquitin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Ubiquitin
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC, IHC-Pmore details
  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse, Horse, Cow, Monkey, African green monkey
  • 免疫原

    Recombinant full length protein corresponding to Human Ubiquitin.

  • 特記事項

    This antibody stains the periphery of senile plaques and of neurofibrillary tangles in Alzheimer's disease brain, the Lewy bodies in Parkinson's disease brain, and the Mallory bodies in alcoholic liver disease.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Ubiquitylation
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Normal Goat Serum (ab7481)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7780の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use at an assay dependent concentration.
IHC-P (3)
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

特記事項
ICC
Use at an assay dependent concentration.
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

ターゲット情報

  • 関連性

    Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • 細胞内局在

    Cell Membrane, Cytoplasmic and Nuclear
  • 参照データベース

    • Entrez Gene: 281370 Cow
    • Entrez Gene: 7314 Human
    • Entrez Gene: 22187 Mouse
    • Entrez Gene: 192255 Rat
    • SwissProt: P0CG53 Cow
    • SwissProt: P0CG47 Human
    • SwissProt: P0CG48 Human
    • SwissProt: P62979 Human
    • SwissProt: P62987 Human
    • SwissProt: P62988 Human
    • SwissProt: P0CG49 Mouse
    • SwissProt: P62991 Mouse
    • SwissProt: P0CG51 Rat
    • SwissProt: P62989 Rat
    • Unigene: 356190 Human
    • Unigene: 520348 Human
    • Unigene: 282093 Mouse
    • Unigene: 371592 Mouse
    • Unigene: 110618 Rat
    • Unigene: 1253 Rat
    • Unigene: 3761 Rat
    see all
  • 別名

    • Epididymis secretory protein Li 50 antibody
    • FLJ25987 antibody
    • HEL S 50 antibody
    • MGC8385 antibody
    • Polyubiquitin B antibody
    • RPS 27A antibody
    • RPS27A antibody
    • UBA 52 antibody
    • UBA 80 antibody
    • UBA52 antibody
    • UBA80 antibody
    • UBB antibody
    • UBB_HUMAN antibody
    • UBC antibody
    • UBCEP 1 antibody
    • UBCEP 2 antibody
    • UBCEP1 antibody
    • UBCEP2 antibody
    • Ubiquitin antibody
    • Ubiquitin B antibody
    see all

画像

  • Immunocytochemistry - Anti-Ubiquitin antibody (ab7780)
    Immunocytochemistry - Anti-Ubiquitin antibody (ab7780)Image courtesy of Armen Petrosyan by Abreview.

    ab7780 staining Ubiquitin in Panc-1 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, blocked with 1% serum for 1 hour at 22°C and then incubated with ab7780 at a 1/2000 dilution for 1 hour at 22°C. The secondary was used at a 1/200 dilution.Green - Golgi residental proteins C2GnT-M.Red -Ubiquitin.Blue - nucleus staining by DAPI.

    See Abreview

  • Immunocytochemistry - Anti-Ubiquitin antibody (ab7780)
    Immunocytochemistry - Anti-Ubiquitin antibody (ab7780)Zhao A et al., PLoS One., 2013: 8(2): e56203. Fig 4.; doi: 10.1371/journal.pone.0056203 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    ab7780 staining ubiquitin in cells transfected with FGAMS-EGFP by ICC/IF (immunocytochemistry/immunofluorescence). Cells were fixed with 3.7% methanol-free formaldehyde at 37°C for 15-20 minutes, blocked with 5% goat serum in PBS-T buffer for 30-60 minutes at room temperature. An Alexa Fluor® 594-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody

  • Immunocytochemistry - Anti-Ubiquitin antibody (ab7780)
    Immunocytochemistry - Anti-Ubiquitin antibody (ab7780)

    ICC/IF image of ab7780 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7780, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) ab150113) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody (ab7780)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody (ab7780)

    ab7780 (1/100) staining Ubiquitin in paraffin-embedded brain sections of a patient with Alzheimer's disease.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody (ab7780)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody (ab7780)This image is courtesy of an anonymous Abreview

    ab7780 staining ubiquitin in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/90) for 16 hours at 4°C. A HRP-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ubiquitin antibody (ab7780)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ubiquitin antibody (ab7780)

    ab7780 at a dilution of 1/1000, staining Ubiquitin in neurons (Alexa 488 secondary at 1/2000) on 30µm coronal rat brain (ab29475) tissue sections in free floating IHC (see protocol link for detailed description). Image shows neuronal staining observed with [A] 20x objective and [B] 40x objective.

    NB: No labeling observed following omission of primary antibody.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (226)

ab7780 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7780 は 226 報の論文で使用されています。

  • Zhang XH  et al. Zenglv Fumai Granule protects cardiomyocytes against hypoxia/reoxygenation-induced apoptosis via inhibiting TRIM28 expression. Mol Med Rep 23:N/A (2021). PubMed: 33398366
  • Liu X  et al. FAT10 protects against ischemia-induced ventricular arrhythmia by decreasing Nedd4-2/Nav1.5 complex formation. Cell Death Dis 12:25 (2021). PubMed: 33414395
  • Liu Y  et al. Peripherally misfolded proteins exacerbate ischemic stroke-induced neuroinflammation and brain injury. J Neuroinflammation 18:29 (2021). PubMed: 33472658
  • Wang D  et al. A Regulatory Loop of FBXW7-MYC-PLK1 Controls Tumorigenesis of MYC-Driven Medulloblastoma. Cancers (Basel) 13:N/A (2021). PubMed: 33494392
  • Zhang Z  et al. Kinase GSK3ß functions as a suppressor in colorectal carcinoma through the FTO-mediated MZF1/c-Myc axis. J Cell Mol Med 25:2655-2665 (2021). PubMed: 33533172
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 50 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Ubiquitin antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HAFs)
Permeabilization
Yes - 0.2% TritonX100/PBS
Specification
HAFs
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Apr 18 2022

Western blot abreview for Anti-Ubiquitin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Jul 23 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ubiquitin antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Specification
Brain
Blocking step
Opal blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 May 23 2018

IHC - Wholemount abreview for Anti-Ubiquitin antibody

Good
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (Brain)
Specification
Brain
Read More

MR. Gabriel Luna

Verified customer

投稿 Jan 10 2018

Western blot abreview for Anti-Ubiquitin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue (Vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
100 uM H2O2 for 2 hours
Specification
Vascular smooth muscle cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 May 22 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Ubiquitin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Dr. Armen Petrosyan

Verified customer

投稿 Apr 03 2017

Western blot abreview for Anti-Ubiquitin antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human mast cell)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
10 µg
Specification
Human mast cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Seong Hoon Kim

Verified customer

投稿 Jun 23 2016

Western blot abreview for Anti-Ubiquitin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - other (Mitochondrial fraction from skeletal muscle)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Mitochondrial fraction from skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Feb 29 2016

Western blot abreview for Anti-Ubiquitin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - other (Mitochondrial fraction from skeletal muscle)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Mitochondrial fraction from skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Feb 18 2016

Western blot abreview for Anti-Ubiquitin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (T lymphocyte)
Specification
T lymphocyte
Treatment
No chemical
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Dec 10 2014

1-10 of 50 Abreviews or Q&A

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