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    ubiquitin-antibody-ab19247.pdf

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Anti-Ubiquitin 抗体 (ab19247)

  • Datasheet
  • SDS
Reviews (5)Q&A (2)References (44)

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Western blot - Anti-Ubiquitin antibody (ab19247)
  • Western blot - Anti-Ubiquitin antibody (ab19247)

Key features and details

  • Rabbit polyclonal to Ubiquitin
  • Suitable for: WB
  • Reacts with: Mouse, Human, Saccharomyces cerevisiae
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Ubiquitin antibody [EP296Y] (ab33893)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Ubiquitin antibody
    Ubiquitin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Ubiquitin
  • 由来種

    Rabbit
  • 特異性

    It can identify free ubiquitin as well as ubiquinated proteins. The antibody recognizes polyubiquitin chains more strongly than monoubiquitinated molecules.
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Mouse, Human, Saccharomyces cerevisiae
  • 免疫原

    Full length native protein (purified) corresponding to Cow Ubiquitin conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Ubiquitylation
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • 10x Citrate Buffer pH 6.0 (ab64214)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab19247の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (3)
1/1000 - 1/5000. Predicted molecular weight: 10 kDa.
特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 10 kDa.

ターゲット情報

  • 関連性

    Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • 細胞内局在

    Cell Membrane, Cytoplasmic and Nuclear
  • 参照データベース

    • Entrez Gene: 7314 Human
    • Entrez Gene: 22187 Mouse
    • SwissProt: P0CG47 Human
    • SwissProt: P0CG48 Human
    • SwissProt: P62979 Human
    • SwissProt: P62987 Human
    • SwissProt: P62988 Human
    • SwissProt: P0CG49 Mouse
    • SwissProt: P62991 Mouse
    • Unigene: 356190 Human
    • Unigene: 520348 Human
    • Unigene: 282093 Mouse
    • Unigene: 371592 Mouse
    see all
  • 別名

    • Epididymis secretory protein Li 50 antibody
    • FLJ25987 antibody
    • HEL S 50 antibody
    • MGC8385 antibody
    • Polyubiquitin B antibody
    • RPS 27A antibody
    • RPS27A antibody
    • UBA 52 antibody
    • UBA 80 antibody
    • UBA52 antibody
    • UBA80 antibody
    • UBB antibody
    • UBB_HUMAN antibody
    • UBC antibody
    • UBCEP 1 antibody
    • UBCEP 2 antibody
    • UBCEP1 antibody
    • UBCEP2 antibody
    • Ubiquitin antibody
    • Ubiquitin B antibody
    see all

画像

  • Western blot - Anti-Ubiquitin antibody (ab19247)
    Western blot - Anti-Ubiquitin antibody (ab19247)
    All lanes :

    Lane 1 : HeLa cell lysate (Control, untreated)
    Lane 2 : HeLa cells treated with MG132 (50 uM for 90 min)
    Lane 3 : Mouse Brain

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 10 kDa



    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes.  ab19247 and ab8245 (loading control to GAPDH) were diluted 1/200  and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging using the Licor Odyssey CLx.

  • Western blot - Anti-Ubiquitin antibody (ab19247)
    Western blot - Anti-Ubiquitin antibody (ab19247)
    Anti-Ubiquitin antibody (ab19247) at 1/1000 dilution + Heat-shocked HeLa cell lysate

    Predicted band size: 10 kDa

プロトコール

  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (44)

ab19247 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab19247 は 44 報の論文で使用されています。

  • Panagaki D  et al. Nuclear envelope budding is a response to cellular stress. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34290138
  • Xu J  et al. Nedd4l downregulation of NRG1 in the mPFC induces depression-like behaviour in CSDS mice. Transl Psychiatry 10:249 (2020). PubMed: 32703967
  • Li Q  et al. The Invasion Plasmid Antigen J (IpaJ) from Salmonella Inhibits NF-?B Activation by Suppressing I?Ba Ubiquitination. Infect Immun 88:N/A (2020). PubMed: 31843963
  • Waizenegger A  et al. Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity. Nat Commun 11:5746 (2020). PubMed: 33184279
  • Wong M  et al. Dynamic Buffering of Extracellular Chemokine by a Dedicated Scavenger Pathway Enables Robust Adaptation during Directed Tissue Migration. Dev Cell 52:492-508.e10 (2020). PubMed: 32059773
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-7 of 7 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Ubiquitin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (spermatocytes)
Permeabilization
No
Specification
spermatocytes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Dec 16 2016

Western blot abreview for Anti-Ubiquitin antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (EC CELLS)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
60 µg
Specification
EC CELLS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More

Abcam user community

Verified customer

投稿 Apr 08 2016

Western blot abreview for Anti-Ubiquitin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse mammary cell)
Loading amount
250 µg
Specification
Mouse mammary cell
Treatment
5ng/ml TGF beta for 8hrs
Gel Running Conditions
Reduced Denaturing (10.0% gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Sep 07 2011

Western blot abreview for Anti-Ubiquitin antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Vero)
Loading amount
30000 cells
Specification
Vero
Treatment
CO-IP for the prot of interest (40kDa)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
Read More

Mr. olivier Reynard

Verified customer

投稿 Jul 27 2006

Immunoprecipitation abreview for Anti-Ubiquitin antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (Saccharomyces cerevisiae)
Total protein in input
5 µg
Specification
Saccharomyces cerevisiae
Immuno-precipitation step
Protein G
Read More

Abcam user community

Verified customer

投稿 Jun 19 2006

Question

Conditions employed for WB testing. Customer is using S.cerevisiae

Read More

Abcam community

Verified customer

Asked on Dec 19 2006

Answer

Thank you for your enquiry. Further to correspondence with the source of this antibody I can tell you that the following approach was employed for the testing of this antibody by western blot analysis. In brief the samples were prepared in denaturing (and what I am assuming, reduced conditions and electrophoresed under denaturing conditions; 1. Cells were washed twice by centrifugation with PBS. Total cell extracts were prepared by suspending cell pellets in RIPA buffer (50mM Tris, pH 7.2, 150mM NaCl, 1% Nonidet P-40, 1% sodium deoxycholate and 0.05% SDS). 2. Proteins were denatured by heating to 95 C in SDS-reducing buffer and were separated by electrophoresis on 12.5% SDS-polyacrylamide gels. The proteins were electrophoretically transferred to Hybond-ECL nitrocellulose membranes. 3. Membranes were probed with ab19247 diluted 1:1,000, and bound antibody was detected using an ECL Western blotting analysis system (Amersham Pharmacia Biotech). A band at approx 10 kDa is detected. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Dec 20 2006

Question

Does this antibody detect both mono- and poly-ubiquitinated proteins?

Read More

Abcam community

Verified customer

Asked on Apr 04 2006

Answer

Thank you for your enquiry. It has not been determined whether ab14372 detects mono- or poly-ubiquitinated proteins. It would quite likely recognize both. Ab19247 should recognize ubiquinated proteins in Western blots regardless of how may ubiquitin chains are present. I hope this information helps. Please let us know if you need anything else.

Read More

Abcam Scientific Support

Answered on Apr 04 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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