Key features and details
- Mouse monoclonal [1B4-UB] to Ubiquitin
- Suitable for: IHC-P, IHC-Fr, WB, IHC-FoFr, ELISA, Flow Cyt
- Reacts with: Cow, Human
- Isotype: IgG1
製品名Anti-Ubiquitin antibody [1B4-UB]
Ubiquitin 一次抗体 製品一覧
製品の詳細Mouse monoclonal [1B4-UB] to Ubiquitin
アプリケーション適用あり: IHC-P, IHC-Fr, WB, IHC-FoFr, ELISA, Flow Cytmore details
種交差性交差種: Cow, Human
Ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin
- Human hypophysis
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.1% Sodium azide
Concentration information loading...
精製度Protein G purified
特記事項（精製）Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Our Abpromise guarantee covers the use of ab122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000.|
|IHC-Fr||1/500 - 1/1000.|
|WB||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||1/10 - 1/50.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
関連性Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
細胞内局在Cell Membrane, Cytoplasmic and Nuclear
- Epididymis secretory protein Li 50 antibody
- FLJ25987 antibody
- HEL S 50 antibody
ab122 は 2 報の論文で使用されています。
- Shevade S et al. Food vacuole associated enolase in plasmodium undergoes multiple post-translational modifications: evidence for atypical ubiquitination. PLoS One 8:e72687 (2013). PubMed: 24009698
- Gao Z et al. Sirtuin 1 (SIRT1) Protein Degradation in Response to Persistent c-Jun N-terminal Kinase 1 (JNK1) Activation Contributes to Hepatic Steatosis in Obesity. J Biol Chem 286:22227-34 (2011). WB ; Human . PubMed: 21540183