Anti-UBF1 抗体 [EPR2742] - BSA and Azide free (ab247745)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2742] to UBF1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-UBF1 antibody [EPR2742] - BSA and Azide free
UBF1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2742] to UBF1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
ab247745 is the carrier-free version of ab109011.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2742 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab247745の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 minutes. |
特記事項 |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 minutes. |
ターゲット情報
-
機能
Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. -
配列類似性
Contains 6 HMG box DNA-binding domains. -
翻訳後修飾
Phosphorylated and activated by PIK3CA. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
-
参照データベース
- Entrez Gene: 7343 Human
- Entrez Gene: 21429 Mouse
- Entrez Gene: 25574 Rat
- Omim: 600673 Human
- SwissProt: P17480 Human
- SwissProt: P25976 Mouse
- SwissProt: P25977 Rat
- Unigene: 89781 Human
see all -
別名
- 90 kDa nucleolus organizer region autoantigen antibody
- Autoantigen NOR-90 antibody
- NOR 90 antibody
see all
画像
-
All lanes : Anti-UBF1 antibody [EPR2742] (ab109011) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 89 kDaThis data was developed using ab109011, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab247745 は論文での使用が確認できていません。