Anti-Ube4a 抗体 [EPR7331] - BSA and Azide free (ab248191)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7331] to Ube4a - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Ube4a antibody [EPR7331] - BSA and Azide free
Ube4a 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7331] to Ube4a - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
ab248191 is the carrier-free version of ab128861.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 1.91 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7331 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Conjugation kits
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248191の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 125 kDa (predicted molecular weight: 123 kDa).
|
|
ICC/IF |
Use at an assay dependent concentration.
|
特記事項 |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 125 kDa (predicted molecular weight: 123 kDa). |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. -
配列類似性
Belongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain. - Information by UniProt
-
参照データベース
- Entrez Gene: 9354 Human
- Entrez Gene: 140630 Mouse
- Entrez Gene: 315608 Rat
- Omim: 603753 Human
- SwissProt: Q14139 Human
- SwissProt: E9Q735 Mouse
- SwissProt: Q6P7A2 Rat
- Unigene: 602967 Human
see all -
別名
- E4 antibody
- KIAA0126 antibody
- MGC133315 antibody
see all
画像
-
All lanes : Anti-Ube4a antibody [EPR7331] (ab128861) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 123 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?This data was developed using ab128861, the same antibody clone in a different buffer formulation.
-
This data was developed using ab128861, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab248191 は論文での使用が確認できていません。