Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7331] to Ube4a - BSA and Azide free
- Suitable for: ICC, Flow Cyt, WB
- Reacts with: Human
製品名Anti-Ube4a antibody [EPR7331] - BSA and Azide free
Ube4a 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7331] to Ube4a - BSA and Azide free
アプリケーション適用あり: ICC, Flow Cyt, WBmore details
適用なし: IHC-P or IP
交差が予測される動物種: Mouse, Rat
Synthetic peptide within Human Ube4a aa 1-100. The exact sequence is proprietary.
Database link: Q14139
Ab248191 is the carrier-free version of ab128861. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248191 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 1.91 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 125 kDa (predicted molecular weight: 123 kDa).|
機能Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
配列類似性Belongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain.
- Information by UniProt
- E4 antibody
- KIAA0126 antibody
- MGC133315 antibody
All lanes : Anti-Ube4a antibody [EPR7331] (ab128861) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 123 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
This data was developed using ab128861, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.