Anti-UBE3C 抗体 (ab101512)
Key features and details
- Rabbit polyclonal to UBE3C
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-UBE3C antibody
UBE3C 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UBE3C -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Recombinant fragment, corresponding to a region within amino acids 164-357 of Human UBE3C isoform 2 (AAH14029).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101512の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 124 kDa.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 124 kDa. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo. -
組織特異性
Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 IQ domain. -
ドメイン
The C-terminal is necessary and sufficient for the poly-ubiquitin chain assembly. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9690 Human
- Entrez Gene: 100763 Mouse
- Omim: 614454 Human
- SwissProt: Q15386 Human
- SwissProt: Q80U95 Mouse
- Unigene: 118351 Human
- Unigene: 137746 Mouse
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別名
- HectH2 antibody
- KIAA0010 antibody
- KIAA10 antibody
see all
画像
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Western blot - Anti-UBE3C antibody (ab101512)Anti-UBE3C antibody (ab101512) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 124 kDa
7.5% SDS-PAGE
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab101512 は 3 報の論文で使用されています。
- Hang C et al. Oncogenic UBE3C promotes breast cancer progression by activating Wnt/ß-catenin signaling. Cancer Cell Int 21:25 (2021). PubMed: 33407510
- Flack JE et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). PubMed: 28689657
- Byrne R et al. Activity-Based Probes for HECT E3 Ubiquitin Ligases. Chembiochem 18:1415-1427 (2017). PubMed: 28425671