リコンビナントRabMAb

Anti-UBE3A 抗体 [EPR23077-14] - BSA and Azide free (ab272173)

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Immunocytochemistry/ Immunofluorescence - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
  • ELISA - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
  • Flow Cytometry - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
  • Immunoprecipitation - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
  • Immunocytochemistry/ Immunofluorescence - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
  • Flow Cytometry - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
  • Immunoprecipitation - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
  • Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23077-14] to UBE3A - BSA and Azide free
  • Suitable for: WB, ICC/IF, ELISA, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free
    UBE3A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23077-14] to UBE3A - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IP
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, K562, HepG2,A549, HEK-293T, PC-12, PC-12 (treated with 10 uM MG-132 for 4 hours), RAW 264.7 and RAW 264.7 (treated with 10 uM MG-132 for 4 hours) whole cell lysates; Mouse spleen tissue lysate;, Rat brain tissue lysate. ICC/IF: HeLa and RAW 264.7 cells. Flow Cyt: HeLa and RAW 264.7 cells. IP: K562 and RAW 264.7 whole cell lysates.

  • 特記事項

    ab272173 is the carrier-free version of ab272168. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ELISA
Recombinant fragment
Flow Cyt
Mouse
ICC/IF
Mouse
IP
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 10, 37 kDa (predicted molecular weight: 100 kDa).
ICC/IF
Use at an assay dependent concentration.
ELISA
Use a concentration of 1 µg/ml.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 10, 37 kDa (predicted molecular weight: 100 kDa).
ICC/IF
Use at an assay dependent concentration.
ELISA
Use a concentration of 1 µg/ml.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IHC-P.

    • ターゲット情報

    • 機能

      E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).

    • パスウェイ

      Protein modification; protein ubiquitination.

    • 関連疾患

      Angelman syndrome

    • 配列類似性

      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.

    • 翻訳後修飾

      Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells.

    • 細胞内局在

      Nucleus. Cytoplasm.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • ANCR antibody
      • Angelman syndrome antibody
      • AS antibody
      • CTCL tumor antigen se37 2 antibody
      • CTCL tumor antigen se37-2 antibody
      • E6 AP antibody
      • E6AP antibody
      • E6AP ubiquitin protein ligase antibody
      • E6AP ubiquitin-protein ligase antibody
      • EPVE6AP antibody
      • FLJ26981 antibody
      • HECT-type ubiquitin transferase E3A antibody
      • HPVE6A antibody
      • Human papilloma virus E6 associated protein Angelman syndrome antibody
      • Human papilloma virus E6 associated protein antibody
      • Human papillomavirus E6-associated protein antibody
      • NY REN 54 antigen antibody
      • Oncogenic protein associated protein E6 AP antibody
      • Oncogenic protein associated protein E6AP antibody
      • Oncogenic protein-associated protein E6-AP antibody
      • Renal carcinoma antigen NY REN 54 antibody
      • Renal carcinoma antigen NY-REN-54 antibody
      • UBE 3A antibody
      • Ube3a antibody
      • UBE3A protein antibody
      • UBE3A_HUMAN antibody
      • Ubiquitin protein ligase E3A antibody
      • Ubiquitin-protein ligase E3A antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
      Immunocytochemistry/ Immunofluorescence - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling UBE3A with ab272168 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weak cytoplasmic staining in HeLa cell line ab195889. Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

    • ELISA - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
      ELISA - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
      indirect ELISA using ab272168 at varying antibody concentrations (1000-0 ng/ml) and Human UBE3A antigen at 1000 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as a secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

    • Flow Cytometry - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
      Flow Cytometry - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

      Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling UBE3A with ab272168 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

    • Immunoprecipitation - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
      Immunoprecipitation - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

      UBE3A was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab272168 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272168 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug

      Lane 2: ab272168 IP in K-562 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272168 in K-562 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 15 seconds

      A 37 kDa degraded band is observed.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

    • Immunocytochemistry/ Immunofluorescence - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
      Immunocytochemistry/ Immunofluorescence - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling UBE3A with ab272168 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weak cytoplasmic staining in RAW 264.7 cell line. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

    • Flow Cytometry - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
      Flow Cytometry - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

      Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cells labelling UBE3A with ab272168 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

    • Immunoprecipitation - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
      Immunoprecipitation - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

      UBE3A was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) (treated with 10 μM MG-132 for 4 hours) whole cell lysate with ab272168 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272168 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 10 μM MG-132 for 4 hours, whole cell lysate 10 ug

      Lane 2: ab272168 IP in RAW 264.7 treated with 10 μM MG-132 for 4 hours, whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272168 in RAW 264.7 treated with 10 μM MG-132 for 4 hours, whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds

      A 37 kDa degraded band is observed.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

    • Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
      Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272173 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272173 は論文での使用が確認できていません。

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