Anti-UBE3A 抗体 (ab10488)
Key features and details
- Rabbit polyclonal to UBE3A
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-UBE3A antibody
UBE3A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UBE3A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Chicken, Guinea pig, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
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免疫原
Synthetic peptide corresponding to Human UBE3A aa 400-500.
Database link: Q05086 -
ポジティブ･コントロール
- WB: HeLa, HEK293T, mouse NIH/3T3 cells, IP: HeLa.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were affinity purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10488の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).
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| IP |
Use at 1-4 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa). |
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IP
Use at 1-4 µg/mg of lysate. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990). -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Angelman syndrome -
配列類似性
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. -
翻訳後修飾
Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 7337 Human
- Entrez Gene: 22215 Mouse
- Omim: 601623 Human
- SwissProt: Q05086 Human
- SwissProt: O08759 Mouse
- Unigene: 598862 Human
- Unigene: 9002 Mouse
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別名
- ANCR antibody
- Angelman syndrome antibody
- AS antibody
see all
画像
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Western blot - Anti-UBE3A antibody (ab10488)All lanes : Anti-UBE3A antibody (ab10488) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 105 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-UBE3A antibody (ab10488)Immunoprecipitation of HeLa UBE3A with ab10488
Lane 1: ab10488
Lane 2: rabbit anti-UBE6A antibody which recognizes an upstream epitope
Lane 3: Control IgG
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab10488 は 1 報の論文で使用されています。
- Lawson-Yuen A et al. Ube3a mRNA and protein expression are not decreased in Mecp2R168X mutant mice. Brain Res 1180:1-6 (2007). PubMed: 17936729