Anti-Ube2N / Ubc13 抗体 [EPR5163] (ab109294)
![Western blot - Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)](https://www.abcam.co.jp/ps/products/109/ab109294/Images/ab109294-122041-anti-ube2n-ubc13-antibody-epr5163-western-blot.jpg "Western blot - Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5163] to Ube2N / Ubc13
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Ube2N / Ubc13 antibody [EPR5163]
Ube2N / Ubc13 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5163] to Ube2N / Ubc13 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Jurkat, HeLa, Raji and 293T cell lysates Flow Cyt (intra): Jurkat cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5163 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109294の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
ターゲット情報
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機能
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the ubiquitin-conjugating enzyme family. -
翻訳後修飾
Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 7334 Human
- Entrez Gene: 93765 Mouse
- Omim: 603679 Human
- SwissProt: P61088 Human
- SwissProt: P61089 Mouse
- Unigene: 524630 Human
- Unigene: 371667 Mouse
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別名
- Bendless like ubiquitin conjugating enzyme antibody
- Bendless-like ubiquitin-conjugating enzyme antibody
- BLU antibody
see all
画像
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Western blot - Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)All lanes : Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 17 kDa
Observed band size: 17 kDa -
![Flow Cytometry (Intracellular) - Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)](https://www.abcam.co.jp/ps/products/109/ab109294/Images/ab109294-122040-anti-ube2n-ubc13-antibody-epr5163-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)Intracellular flow cytometric analysis of Ube2N / Ubc13 in permeabilized Jurkat cells, using ab109294 at a dilution of 1/250 (red) or a rabbit IgG (negative) (green).
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![Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)](https://www.abcam.co.jp/ps/products/109/ab109294/Images/ab109294-3-benefits-of-recombinant-antibodies.png)
Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)
データシートおよび資料
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Datasheet download
参考文献 (0)
ab109294 は論文での使用が確認できていません。
