Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5334] to UBE2M/UBC12 - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-UBE2M/UBC12 antibody [EPR5334] - BSA and Azide free
UBE2M/UBC12 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5334] to UBE2M/UBC12 - BSA and Azide free
アプリケーション適用あり: ICC, WBmore details
適用なし: Flow Cyt,IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human UBE2M/UBC12 aa 50-150. The exact sequence is proprietary.
ab248068 is the carrier-free version of ab124946 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248068 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as UBE2M
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.|
機能Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
パスウェイProtein modification; protein neddylation.
配列類似性Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
ドメインBoth the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
- Information by UniProt
- hUbc12 antibody
- NEDD8 carrier protein antibody
- NEDD8 conjugating enzyme Ubc12 antibody
All lanes : Anti-UBE2M/UBC12 antibody [EPR5334] (ab124946) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21 kDa
This data was developed using ab124946, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.