Anti-UBE2C 抗体 (ab3935)

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Key features and details

  • Goat polyclonal to UBE2C
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21.5 kDa).

A 1 hour primary incubation is recommended for this product.
IP Use at an assay dependent concentration. (See Abreview)

ターゲット情報

  • 機能

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 配列類似性

    Belongs to the ubiquitin-conjugating enzyme family.

  • 翻訳後修飾

    Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cyclin selective ubiquitin carrier protein antibody
      • dJ447F3.2 antibody
      • Mitotic specific ubiquitin conjugating enzyme antibody
      • UB E2C antibody
      • UBCH 10 antibody
      • UbcH10 antibody
      • UBE 2C antibody
      • Ube2c antibody
      • UBE2C_HUMAN antibody
      • Ubiquitin carrier protein C antibody
      • Ubiquitin carrier protein E2 C antibody
      • Ubiquitin carrier protein E2C antibody
      • Ubiquitin conjugating enzyme E2 C antibody
      • Ubiquitin conjugating enzyme E2C antibody
      • Ubiquitin protein ligase C antibody
      • Ubiquitin-conjugating enzyme E2 C antibody
      • Ubiquitin-protein ligase C antibody
      see all

    画像

    • Western blot - Anti-UBE2C antibody (ab3935)
      Western blot - Anti-UBE2C antibody (ab3935)

      ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):

      Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
      Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
      Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
      Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).

      For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.

      ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):

      Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
      Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
      Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
      Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).

      For a more detailed descri
    • Immunoprecipitation - Anti-UBE2C antibody (ab3935)
      Immunoprecipitation - Anti-UBE2C antibody (ab3935)

      ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (106 cells/ml) using 40µl of ab3935 saturated protein G dynabeads, by Western blot (ECL):

      Lane 1 = IP from Noc arrested HeLa cell extract (beads only).
      Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).
      Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)
      Lane 4 = Noc arrested HeLa whole cell lysate.

      For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.

    参考文献 (3)

    ab3935 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3935 は 3 報の論文で使用されています。

    • Zhang Y  et al. Inhibition of microRNA-17/20a suppresses cell proliferation in gastric cancer by modulating UBE2C expression. Oncol Rep 33:2529-36 (2015). PubMed: 25760688
    • Argenta PA  et al. Predicting response to the anti-estrogen fulvestrant in recurrent ovarian cancer. Gynecol Oncol N/A:N/A (2013). PubMed: 23911795
    • Ieta K  et al. Identification of overexpressed genes in hepatocellular carcinoma, with special reference to ubiquitin-conjugating enzyme E2C gene expression. Int J Cancer 121:33-8 (2007). PubMed: 17354233

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