Anti-UBE2C 抗体 (ab3935)

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Western blot - Anti-UBE2C antibody (ab3935)
  • Immunoprecipitation - Anti-UBE2C antibody (ab3935)

Key features and details

  • Goat polyclonal to UBE2C
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-UBE2C antibody [EPR23165-31] (ab252940)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-UBE2C antibody
    UBE2C 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to UBE2C

  • 由来種

    Goat

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide: QETYSKQVTSQEP, corresponding to C terminal amino acids 167-179 of Human UBE2C.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21.5 kDa).

A 1 hour primary incubation is recommended for this product.
IP Use at an assay dependent concentration. (See Abreview)

ターゲット情報

  • 機能

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 配列類似性

    Belongs to the ubiquitin-conjugating enzyme family.

  • 翻訳後修飾

    Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cyclin selective ubiquitin carrier protein antibody
      • dJ447F3.2 antibody
      • Mitotic specific ubiquitin conjugating enzyme antibody
      • UB E2C antibody
      • UBCH 10 antibody
      • UbcH10 antibody
      • UBE 2C antibody
      • Ube2c antibody
      • UBE2C_HUMAN antibody
      • Ubiquitin carrier protein C antibody
      • Ubiquitin carrier protein E2 C antibody
      • Ubiquitin carrier protein E2C antibody
      • Ubiquitin conjugating enzyme E2 C antibody
      • Ubiquitin conjugating enzyme E2C antibody
      • Ubiquitin protein ligase C antibody
      • Ubiquitin-conjugating enzyme E2 C antibody
      • Ubiquitin-protein ligase C antibody
      see all

    画像

    • Western blot - Anti-UBE2C antibody (ab3935)
      Western blot - Anti-UBE2C antibody (ab3935)

      ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):

      Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
      Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
      Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
      Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).

      For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.

      ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):

      Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
      Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
      Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
      Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).

      For a more detailed descri
    • Immunoprecipitation - Anti-UBE2C antibody (ab3935)
      Immunoprecipitation - Anti-UBE2C antibody (ab3935)

      ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (106 cells/ml) using 40µl of ab3935 saturated protein G dynabeads, by Western blot (ECL):

      Lane 1 = IP from Noc arrested HeLa cell extract (beads only).
      Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).
      Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)
      Lane 4 = Noc arrested HeLa whole cell lysate.

      For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.

    参考文献 (3)

    ab3935 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3935 は 3 報の論文で使用されています。

    • Zhang Y  et al. Inhibition of microRNA-17/20a suppresses cell proliferation in gastric cancer by modulating UBE2C expression. Oncol Rep 33:2529-36 (2015). PubMed: 25760688
    • Argenta PA  et al. Predicting response to the anti-estrogen fulvestrant in recurrent ovarian cancer. Gynecol Oncol N/A:N/A (2013). PubMed: 23911795
    • Ieta K  et al. Identification of overexpressed genes in hepatocellular carcinoma, with special reference to ubiquitin-conjugating enzyme E2C gene expression. Int J Cancer 121:33-8 (2007). PubMed: 17354233

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