Anti-UBE2C 抗体 (ab3935)
Key features and details
- Goat polyclonal to UBE2C
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-UBE2C antibody
UBE2C 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to UBE2C -
由来種
Goat -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide: QETYSKQVTSQEP, corresponding to C terminal amino acids 167-179 of Human UBE2C.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3935の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 2.5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21.5 kDa).
A 1 hour primary incubation is recommended for this product. |
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| IP |
Use at an assay dependent concentration. (See Abreview)
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| 特記事項 |
|---|
|
WB
Use a concentration of 2.5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21.5 kDa). A 1 hour primary incubation is recommended for this product. |
|
IP
Use at an assay dependent concentration. (See Abreview) |
ターゲット情報
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機能
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the ubiquitin-conjugating enzyme family. -
翻訳後修飾
Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed. - Information by UniProt
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参照データベース
- Entrez Gene: 11065 Human
- Omim: 605574 Human
- SwissProt: O00762 Human
- Unigene: 93002 Human
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別名
- Cyclin selective ubiquitin carrier protein antibody
- dJ447F3.2 antibody
- Mitotic specific ubiquitin conjugating enzyme antibody
see all
画像
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Western blot - Anti-UBE2C antibody (ab3935)
ab3935 at a 2.5
µ g/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2
ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):µ l).
Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µ l).
Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µ l).
Lane 4 = Asynchronous HeLa cell whole cell lysate (20µ l).
For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.
Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).
For a more detailed descri -
Immunoprecipitation - Anti-UBE2C antibody (ab3935)ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (106 cells/ml) using 40
µ l of ab3935 saturated protein G dynabeads, by Western blot (ECL):Lane 1 = IP from Noc arrested HeLa cell extract (beads only).
Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).
Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)
Lane 4 = Noc arrested HeLa whole cell lysate.For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab3935 は 3 報の論文で使用されています。
- Zhang Y et al. Inhibition of microRNA-17/20a suppresses cell proliferation in gastric cancer by modulating UBE2C expression. Oncol Rep 33:2529-36 (2015). PubMed: 25760688
- Argenta PA et al. Predicting response to the anti-estrogen fulvestrant in recurrent ovarian cancer. Gynecol Oncol N/A:N/A (2013). PubMed: 23911795
- Ieta K et al. Identification of overexpressed genes in hepatocellular carcinoma, with special reference to ubiquitin-conjugating enzyme E2C gene expression. Int J Cancer 121:33-8 (2007). PubMed: 17354233
