Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MILR42872] to UBA3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
製品名Anti-UBA3 antibody [MILR42872] - BSA and Azide free
UBA3 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [MILR42872] to UBA3 - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human UBA3. The exact sequence is proprietary.
ab247984 is the carrier-free version of ab124728 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247984 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as UBE1C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.|
機能Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
パスウェイProtein modification; protein neddylation.
配列類似性Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.
- Information by UniProt
- DKFZp566J164 antibody
- EC 6.3.2. antibody
- hUba3 antibody
All lanes : Anti-UBA3 antibody [MILR42872] (ab124728)
Lane 1 : Fetal lung cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : RAW 264.7 cell lysate
Lane 5 : HepG2 cell lysate
Lane 6 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa
This data was developed using ab124728, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.