Anti-UBA3 抗体 [MILR42872] - BSA and Azide free (ab247984)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MILR42872] to UBA3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-UBA3 antibody [MILR42872] - BSA and Azide free
UBA3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [MILR42872] to UBA3 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab247984 is the carrier-free version of ab124728.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
MILR42872 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab247984の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa. |
ターゲット情報
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機能
Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. -
組織特異性
Ubiquitously expressed. -
パスウェイ
Protein modification; protein neddylation. -
配列類似性
Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. - Information by UniProt
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参照データベース
- Entrez Gene: 9039 Human
- Entrez Gene: 22200 Mouse
- Entrez Gene: 117553 Rat
- Omim: 603172 Human
- SwissProt: Q8TBC4 Human
- SwissProt: Q8C878 Mouse
- SwissProt: Q99MI7 Rat
- Unigene: 154320 Human
see all -
別名
- DKFZp566J164 antibody
- EC 6.3.2. antibody
- hUba3 antibody
see all
画像
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All lanes : Anti-UBA3 antibody [MILR42872] (ab124728)
Lane 1 : Fetal lung cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : RAW 264.7 cell lysate
Lane 5 : HepG2 cell lysate
Lane 6 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDaThis data was developed using ab124728, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab247984 は論文での使用が確認できていません。