Anti-UAP1 抗体 (ab95949)
Key features and details
- Rabbit polyclonal to UAP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-UAP1 antibody
UAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UAP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Zebrafish
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免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 53-473 of UAP1 isoform AGX1 (Human)
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ポジティブ･コントロール
- H1299 lysate, A549 cells (IF), and ES-2 xenograft (IHC)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95949の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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| IHC-P |
1/100 - 1/500.
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| ICC/IF |
1/100 - 1/200.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
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IHC-P
1/100 - 1/500. |
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ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P. -
組織特異性
Widely expressed. Isoform AGX1 is more abundant in testis than isoform AGX2, while isoform AGX2 is more abundant than isoform AGX1 in somatic tissue. Expressed at low level in placenta, muscle and liver. -
パスウェイ
Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1. -
配列類似性
Belongs to the UDPGP type 1 family. -
細胞内局在
Cytoplasm. In spermatozoa, localized to the principal piece of the tail, the neck region of the head and to a lesser extent, the midpiece of the tail. - Information by UniProt
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参照データベース
- Entrez Gene: 535325 Cow
- Entrez Gene: 6675 Human
- Entrez Gene: 107652 Mouse
- Omim: 602862 Human
- SwissProt: Q16222 Human
- SwissProt: Q91YN5 Mouse
- Unigene: 492859 Human
- Unigene: 27969 Mouse
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別名
- AGX 1 antibody
- AGX antibody
- AGX-1 antibody
see all
画像
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Western blot - Anti-UAP1 antibody (ab95949)Anti-UAP1 antibody (ab95949) at 1/3000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 57 kDa
gel concentration: 7.5% -
Immunocytochemistry/ Immunofluorescence - Anti-UAP1 antibody (ab95949)Immunofluorescence analysis of paraformaldehyde fixed A549 cells using ab95949: (1) at 1/200 dilution, (2) merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody (ab95949)Immunohistochemical analysis of paraffin embedded ES 2 xenograft using ab95949 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab95949 は 1 報の論文で使用されています。
- Mason B et al. Fbxl17 is rearranged in breast cancer and loss of its activity leads to increased global O-GlcNAcylation. Cell Mol Life Sci 77:2605-2620 (2020). PubMed: 31560077