Anti-UACA/Nucling 抗体 (ab99323)
Key features and details
- Rabbit polyclonal to UACA/Nucling
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-UACA/Nucling antibody
UACA/Nucling 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UACA/Nucling -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Cow, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human UACA/Nucling aa 1050-1150.
Database link: NP_060473.2 -
ポジティブ･コントロール
- WB: HeLa, 293T, TCMK-1, C26.WT, C6 whole cell lysates IP: HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99323の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 162 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 162 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.
Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury. -
組織特異性
Highly expressed in skeletal muscle, heart, kidney and pancreas. Expressed in choroid, retina and epidermal melanocytes. Expressed in eye muscles and thyroid follicular cells. -
配列類似性
Contains 6 ANK repeats. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton. Expressed diffusely in cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 281559 Cow
- Entrez Gene: 55075 Human
- Entrez Gene: 72565 Mouse
- Entrez Gene: 315732 Rat
- Omim: 612516 Human
- SwissProt: Q8HYY4 Cow
- SwissProt: Q9BZF9 Human
- SwissProt: Q8CGB3 Mouse
see all -
別名
- FLJ10128 antibody
- KIAA1561 antibody
- MGC141967 antibody
see all
画像
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Western blot - Anti-UACA/Nucling antibody (ab99323)All lanes : Anti-UACA/Nucling antibody (ab99323) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 162 kDa
Exposure time: 10 seconds -
Western blot - Anti-UACA/Nucling antibody (ab99323)All lanes : Anti-UACA/Nucling antibody (ab99323) at 0.1 µg/ml
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 3 : 4T1 (Mouse mammary gland carcinoma cell line) whole cell lysate
Lane 4 : C26.WT
Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 162 kDa
Exposure time: 3 minutesDetection: chemiluminescence.
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Immunoprecipitation - Anti-UACA/Nucling antibody (ab99323)Detection of UACA/Nucling in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded), using ab99323 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab99323 は論文での使用が確認できていません。
