U0126, Selective MKK inhibitor (ab120241)
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製品の概要
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製品名U0126, Selective MKK inhibitor
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製品の詳細Selective MKK inhibitor
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生理活性の詳細Selective non-competitive inhibitor of MAP kinase kinase (MKK). Inhibits MKK1, MKK2 (IC50 values of 0.07 and 0.06 μM, respectively) and MKK5 with little or no effect on a wide range of other kinases. Centrally active following systemic administration in vivo.
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精製度> 98%
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CAS 番号109511-58-2
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構造式
製品の特性
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体系名1,4-Diamino-2,3-dicyano-1,4-bis(2-aminophenylthio)butadiene
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分子量380.49
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分子式C18H16N6S2
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PubChem 登録番号3006531
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保存方法Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
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溶解性Soluble in DMSO to 100 mM
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使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記N/C(Sc1ccccc1N)=C(/C#N)C(C#N)=C(/N)Sc2ccccc2N
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由来
Synthetic
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研究分野
関連製品
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Related Products
画像
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Western blot - U0126, Selective MKK inhibitor (ab120241)Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.
Lane 1: Marker
Lane 2: A431 lysate starved
Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)
Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution
Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.
Observed bands:
RSK1p90 (pS380) band size: 83 kDa
AKT1 (pS473) band size: 56 kDa
ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
RPS6 (pS235/236) band size: 31 kDa
Rab11a band size: 24 kDa
Unknown band: 130kDa
Performed under reducing conditions; Samples l
参考文献
This product has been referenced in:
- Bain J et al. The selectivity of protein kinase inhibitors: a further update. Biochem J 408:297-315 (2007). Read more (PubMed: 17850214) »
- Favata MF et al. Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J Biol Chem 273:18623-32 (1998). Read more (PubMed: 9660836) »
- Duncia JV et al. MEK inhibitors: the chemistry and biological activity of U0126, its analogs, and cyclization products. Bioorg Med Chem Lett 8:2839-44 (1998). Read more (PubMed: 9873633) »
