Anti-Tyrosine Hydroxylase 抗体 - Neuronal Marker (ab112)
Key features and details
- Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Tyrosine Hydroxylase antibody - Neuronal Marker
Tyrosine Hydroxylase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mammals
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免疫原
Full length SDS denatured protein (purified from pheochromocytoma) (Rat).
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特記事項
ab112 can be used as a marker for dopaminergic and noradrenergic neurons.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.50
Constituents: 0.01% BSA, 0.87% Sodium chloride, 50% Glycerol, 0.238% HEPES -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/750. | |
| WB | 1/200. Predicted molecular weight: 60 kDa. |
ターゲット情報
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機能
Plays an important role in the physiology of adrenergic neurons. -
組織特異性
Mainly expressed in the brain and adrenal glands. -
パスウェイ
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
関連疾患
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
配列類似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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参照データベース
- Entrez Gene: 25085 Rat
- SwissProt: P04177 Rat
- Unigene: 11082 Rat
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別名
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
画像
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Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Predicted band size: 60 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)Paraffin embedded sections of rat brain tissue were stained for Tyrosine Hydroxylase with ab112 at 1/5000 dilution in immunohistochemical analysis (Panel 1).
Panel 2 shows an image in which ab112 was replaced with a Rabbit IgG1 isotype control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)This image is courtesy of an Abreview submitted by Dr Guillermo Estivill-Torrusab112 at 1/500 staining rat brain tissue (ab29475) sections by IHC-P.
The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 14 hours. A biotinylated swine anti-rabbit IgG was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)This image is courtesy of an anonymous Abreviewab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry.
The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.
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Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + rat caudate lysate at 10 µg
Predicted band size: 60 kDa
プロトコール
参考文献 (179)
ab112 は 179 報の論文で使用されています。
- Nullmeier S et al. Glutamic acid decarboxylase 67 haplodeficiency in mice: consequences of postweaning social isolation on behavior and changes in brain neurochemical systems. Brain Struct Funct 225:1719-1742 (2020). PubMed: 32514634
- Zhou L et al. Colocalization of dopamine receptors in BDNF-expressing peptidergic neurons in the paraventricular nucleus of rats. J Chem Neuroanat 106:101794 (2020). PubMed: 32315740
- You MJ et al. Human umbilical cord-derived mesenchymal stem cells alleviate schizophrenia-relevant behaviors in amphetamine-sensitized mice by inhibiting neuroinflammation. Transl Psychiatry 10:123 (2020). PubMed: 32341334
- Koga K et al. Ascending noradrenergic excitation from the locus coeruleus to the anterior cingulate cortex. Mol Brain 13:49 (2020). PubMed: 32216807
- Cao S et al. Fast Localization and Sectioning of Mouse Locus Coeruleus. Biomed Res Int 2020:4860735 (2020). PubMed: 32190666
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