Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase
- Suitable for: WB, Flow Cyt, ICC, IHC-P
- Reacts with: Mouse, Rat, Human
製品名Anti-Tyrosine Hydroxylase antibody [EP1533Y]
Tyrosine Hydroxylase 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase
アプリケーション適用あり: WB, Flow Cyt, ICC, IHC-Pmore details
種交差性交差種: Mouse, Rat, Human
- WB: Human adrenal gland lysate. SH-SY5Y whole cell lysate. Mouse and rat brain lysate. PC-12 whole cell lysate. IHC-P: Human and mouse cerebral cortex tissue, Rat and mouse brain tissue. ICC: Neuro-2a and mouse midbrain neuron cells. Flow Cyt: SH-SY5Y cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度Protein A purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab75875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 59 kDa.Can be blocked with Tyrosine Hydroxylase peptide (ab183303).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/1500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
機能Plays an important role in the physiology of adrenergic neurons.
組織特異性Mainly expressed in the brain and adrenal glands.
パスウェイCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
関連疾患Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
配列類似性Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
- Information by UniProt
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
All lanes : Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875) at 1/1000 dilution (Purified)
Lane 1 : Human adrenal gland lysate
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
Immunohistochemical analysis of rat brain tissue section labeling Tyrosine Hydroxylase with ab75875 at 1/1000 dilution. The tissue was fixed with Formaldehyde. Antigen retrieval was heat mediated using citric acid. A biotin conjugated goat anti-rabbit polyclonal IgG at 1/250 was used as the secondary antibody.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse midbrain neuron cells labelling Tyrosine Hydroxylase with ab75875 at 1/10 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in mouse primary midbrain neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).
Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Tyrosine Hydroxylase with purified ab75875 at 1/50 dilution (2.18 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue sections labeling Tyrosine Hydroxylase with purified ab75875 at 1/1500 dilution (0.07 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Tyrosine Hydroxylase with purified ab75875 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemical analysis of mouse brain tissue section labeling Tyrosine Hydroxylase with ab75875 at 1/800 dilution. The tissue was fixed with Formaldehyde. Antigen retrieval was heat mediated using citric acid. A biotin conjugated goat anti-rabbit polyclonal IgG at 1/250 was used as the secondary antibody.
ab75875 は 23 報の論文で使用されています。
- Chen X et al. Entorhinal cortex-based metabolic profiling of chronic restraint stress mice model of depression. Aging (Albany NY) 12:3042-3052 (2020). PubMed: 32074509
- Fan YN et al. Characterization of Group I Metabotropic Glutamate Receptors in Rat and Human Adrenal Glands. Front Physiol 11:401 (2020). PubMed: 32536873
- Zhong ZF et al. Neuroprotective Effects of Salidroside on Cerebral Ischemia/Reperfusion-Induced Behavioral Impairment Involves the Dopaminergic System. Front Pharmacol 10:1433 (2019). PubMed: 31920641
- Zhang L et al. Semaglutide is Neuroprotective and Reduces a-Synuclein Levels in the Chronic MPTP Mouse Model of Parkinson's Disease. J Parkinsons Dis 9:157-171 (2019). PubMed: 30741689
- Reeves FA et al. Prostatic nerve subtypes independently predict biochemical recurrence in prostate cancer. J Clin Neurosci 63:213-219 (2019). PubMed: 30772200