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Anti-Tyrosine Hydroxylase 抗体 (ab6211)

  • Datasheet
Reviews (4)Q&A (8)References (41)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
  • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab6211)
  • Western blot - Anti-Tyrosine Hydroxylase antibody (ab6211)
  • Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)

Key features and details

  • Rabbit polyclonal to Tyrosine Hydroxylase
  • Suitable for: IHC-FoFr, WB, ICC/IF, IHC-Fr
  • Reacts with: Mouse, Rat, Guinea pig, Human
  • Isotype: IgG

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関連製品

製品の概要

  • 製品名

    Anti-Tyrosine Hydroxylase antibody
    Tyrosine Hydroxylase 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Tyrosine Hydroxylase
  • 由来種

    Rabbit
  • 特異性

    Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme.
  • アプリケーション

    適用あり: IHC-FoFr, WB, ICC/IF, IHC-Frmore details
  • 種交差性

    交差種: Mouse, Rat, Guinea pig, Human
    交差が予測される動物種: Chicken, Chimpanzee, Macaque monkey
  • 免疫原

    Synthetic peptide corresponding to Rat Tyrosine Hydroxylase aa 32-47 conjugated to Keyhole Limpet Haemocyanin (KLH) (Glutaraldehyde).
    Sequence:

    PRFIGRRQSLIEDARK


    Database link: P04177
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

     

    This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7
    Constituent: Whole serum
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • 一次抗体 備考

    This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Mouse Tyrosine Hydroxylase protein (His tag) (ab219278)
  • Related Products

    • Orexin A (bovine, human, mouse, rat), Neuropeptide involved in feeding and sleep (ab120212)
    • Normal Goat Serum (ab7481)

アプリケーション

Our Abpromise guarantee covers the use of ab6211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 42,53,84 kDa.
ICC/IF 1/1000.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Plays an important role in the physiology of adrenergic neurons.
  • 組織特異性

    Mainly expressed in the brain and adrenal glands.
  • パスウェイ

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • 関連疾患

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • 配列類似性

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Target information above from: UniProt accession P07101 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 7054 Human
    • Entrez Gene: 21823 Mouse
    • Entrez Gene: 25085 Rat
    • Omim: 191290 Human
    • SwissProt: P07101 Human
    • SwissProt: P24529 Mouse
    • SwissProt: P04177 Rat
    • Unigene: 435609 Human
    • Unigene: 1292 Mouse
    • Unigene: 11082 Rat
    see all
  • 別名

    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

画像

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom

    Tyrosine hydroxylase antibody (ab6211) staining rat mesencephalic serotoninergic neurons. Pictures taken using objectives X20 (A) or X20 (B). Note that the staining is observed in the cell body and the processes of these neurons. ab6211 was used at 1/1000 incubated for 2 days at room temperature. Secondary antibody was anti-rabbit Alexa Fluor 488 used at 1/1000 for 2 hours at room temperature. Rat brain tissue (ab29475) was perfusion fixed (paraformaldehyde 4% ) followed by post-fixation overnight in the same fixative, cryoprotected in 20% sucrose and then frozen in OCT. 30µm coronal sections of brain were cutting by cyrostat and immunohistochemistry was performed as the 'free floating' technique.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab6211)
    Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab6211)

    ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • Western blot - Anti-Tyrosine Hydroxylase antibody (ab6211)
    Western blot - Anti-Tyrosine Hydroxylase antibody (ab6211)
    Anti-Tyrosine Hydroxylase antibody (ab6211) at 1/100 dilution + PC12 cell lysates at 6 µg

    Performed under reducing conditions.

    Predicted band size: 42,53,84 kDa



    Three bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa.

  • Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
    Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)Image courtesy of QBM Cell Sciences, Ottawa, Canada
    ab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)

    Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1:100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

プロトコール

  • Recommended IHC-Fr protocol with ab6211

Click here to view the general protocols

データシートおよび資料

    • Datasheet
  • 参考文献 (41)

    ab6211 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6211 は 41 報の論文で使用されています。

    • Yang SY  et al. Glucocerebrosidase activity, cathepsin D and monomeric a-synuclein interactions in a stem cell derived neuronal model of a PD associated GBA1 mutation. Neurobiol Dis 134:104620 (2020). PubMed: 31634558
    • Liu AQ  et al. Sensory nerve-deficient microenvironment impairs tooth homeostasis by inducing apoptosis of dental pulp stem cells. Cell Prolif 53:e12803 (2020). PubMed: 32246537
    • Rosas-Jarquín CJ  et al. Chronic consumption of cassava juice induces cellular stress in rat substantia nigra. Iran J Basic Med Sci 23:93-101 (2020). PubMed: 32405352
    • Han X  et al. Small molecule-driven NLRP3 inflammation inhibition via interplay between ubiquitination and autophagy: implications for Parkinson disease. Autophagy 15:1860-1881 (2019). PubMed: 30966861
    • Lohoff FW  et al. Deletion of the vesicular monoamine transporter 1 (vmat1/slc18a1) gene affects dopamine signaling. Brain Res N/A:N/A (2019). PubMed: 30685272
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 12 Abreviews or Q&A

    Immunohistochemistry free floating abreview for Anti-Tyrosine Hydroxylase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Zebra finch Tissue sections (Brain)
    Specification
    Brain
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 17 2019

    Immunohistochemistry (Resin sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Resin sections)
    Sample
    Mouse Tissue sections (Retina)
    Specification
    Retina
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 27 2016

    Question

    Product code: 6211

    Inquiry: I am currently trying to find a primary antibody for total tyrosine hydroxylase that has an associated blocking peptide available. The primary I have been trialing has no blocking peptide and the band is at an improper location. Does 6211 label total tyrosine hydroxylase and is there a blocking peptide available?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 04 2012

    Answer

    Thank you for contacting Abcam.

    I apologize for the delay, our laboratory was looking into the availability of the blocking peptide for ab6211.

    We do not have the blocking peptide for ab6211 available separately. However, the peptide sequence is published: PRFIGRRQSLIEDARK, should you wish to pursue your own means of synthesizing the peptide. A 80-85% purity is generally good enough for blocking studies. Below is a link with a list of companies that provide peptide synthesis services:
    http://www.peptideresource.com/custom-peptide.html

    ab6211 recognizes amino acids 32-47 of Rat Tyrosine Hydroxylase, and is also reactive with mouse, guinea pig, and human tyrosine hydroxylase with predicted reactivity in chicken, chimpanzee, and macaque monkey. Thus, it should label total tyrosine hydroxylase through recognition of amino acids 32-47.

    To our knowledge, ab6211 has not been tested in western blot. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

    Simply follow these easy steps below to apply for our AbTrial Program:

    1. Reply to this email, letting us know you are interested in testing this product.

    2. Our scientists will email you an inactive personal discount code for the value of the product.

    3. Purchase and test the product at the regular price.

    4. Submit your results, including your discount code in the additional notes section of your Abreview.

    5. Once the Abreview is submitted, the discount code will become active.

    6. Apply your discount code on your next order to receive that value off.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 04 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Brain-SNpc)
    Specification
    Brain-SNpc
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.3% TritonX in 0.1% PBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Apr 13 2012

    Question

    Thanks for your kindly reply, after I contacted with this customer, she would like to have a credit note. Therefore, would you please help this customer to create a credit note? Thanks for your kindly assistance.    

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2011

    Answer

    Thank you for confirming this information and for your help and cooperation with this case. As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order. As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. The reference number, or credit ID, is ******. Please refer to this number in any correspondence with the accounting department. I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.  

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2011

    Question

    LOT NUMBER 1077964 DESCRIPTION OF THE PROBLEM No staining SAMPLE Species: mouse What’s cell line or tissue: brain tissue PRIMARY ANTIBODY Species: rabbiy Reacts against: Tyrosine hydroxylase At what dilution(s) have you tested this antibody: 1:200/1:500/1:1000 Diluent buffer: 0.3% Triton X-100 Incubation time: overnight Incubation temperature: 4 degree What washing steps were done (which buffer, number of washes): PBS wash 3 time, each time 5 min ANTIBODY STORAGE CONDITIONS -20 FIXATION OF SAMPLE Yes or No : yes If yes, Fixative agent and concentration: paraformaldehyde Fixation time: perfusion and post-fix overnight Fixation temperature : 4 degree ANTIGEN RETRIEVAL no PERMEABILIZATION STEP Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers? yes Permeabilizing agent and concentration: PBS-0.1% Triton for 15 min at RT BLOCKING CONDITIONS Concentration: 10% donkey serum/PBS-0.05% Tween Blocking time : 1hr Blocking temperature: RT SECONDARY ANTIBODY Species: Alexa 594 donkey anti-rabbit Reacts against which species: At what dilution(s) have you tested this antibody: 1:200/1:500 Diluent buffer: 0.3% Triton X-100 Incubation time: overnight What washing steps were done (which buffer, number of washes): PBS wash 3 time, each time 5 min ˙Fluorochrome or enzyme conjugate (eg: FITC, HRP, AP, biotin…etc): Alexa 594 donkey anti-rabbit Do you know whether the problems you are experiencing come from the secondary? NO HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 7 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? NO

    Read More

    Abcam community

    Verified customer

    Asked on Oct 24 2011

    Answer

    Thank you for filling in the questionnaire and for reporting this problem to us. We will use the details you sent about your protocol in order to investigate the source of the problem. I have looked through your protocol, which looks fine to me. I appreciate the time that has been spent on these experiments and would be pleased to arrange a credit note or a replacement. Unfortunately the only lot of ab6211 we currently have in stock is the same your customer received. Therefore I can offer a replacement with one of the following alternative products, tested in Mouse and IHC-FoFR : ab41528, www.abcam.com/ab41528 ab51191, www.abcam.com/ab51191 ab112, www.abcam.com/ab112 ab113, www.abcam.com/ab113 I look forward to hearing from you with details of how you would like to proceed. Could you also please inform us that you added glycerol to ab6211 before storing at -20°C as recommended on the datasheet?

    Read More

    Abcam Scientific Support

    Answered on Oct 24 2011

    Question

    I recently purchased one of your products ab6211, a Tyrosine Hydroxylase antibody, and I'm having trouble with doing IHC on dopaminergic neurons. When I did a double staining of these dopaminergic with both ab6211 and the monoclonal anti-tyrosine hydroxlase TH-2 antibody from sigma, I got different results. The sigma antibody showed very specific staining, outlining specific areas of cells, whereas ab6211 showed a lot of aspecific spotty background staining. I used a dilution of 1:1000 (as recommended) for ab6211 and 1:8000 for the sigma antibody. The secondary antibody used for the ab6211 was Cy5 conjugated AffiniPUre Donkey anti-Rabbit IgG from Jackson Labs. I've attached a picture of a confocal image taken of these cells. Red is staining with TH-2, Green is ab6211, blue is DAPI. I would appreciate any advice on what to do to improve IHC staining with ab6211.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 08 2006

    Answer

    Thank you for your enquiry. Since you are seeing some specific staining in addition to this ubiquitous background staining, I am thinking this may be non-specific staining due to using too high a concentration of primary antibody. The recommendation on the datasheet is a starting point only, and I would recommend diluting this antibody out further, perhaps trying serial dilutions 1:2000 and 1:4000. In addition, I didn't see from your enquiry how you were incubating with the antibody. I would recommend an overnight incubation at 4C, as this will give the most specific signal. If you are still having problems with this antibody after following these suggestions, please let us know. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Nov 13 2006

    Question

    In our lab we use a phenol-ethanol supernatant obtained after precipitation of DNA with ethanol, we wonder if we could use this supernatant for the ELISA with acetilcholine and noradrenaline antibodies. The protocols displayed in the internet page, have been usefull. I look forward to your reply.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 01 2006

    Answer

    Thank you for your enquiry. Regarding "using protein in the phenol-ethanol supernatant for ELISA", would you first precipitate the protein? In either case, I'm not sure if the protein would be recognized by the antibodies since we have not tested this ourselves. We invite you to use our Abreview system. The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for rewards such as Amazon.com gift certificates or discounts on future antibody purchases. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. I hope this information helps and please let me know how the ELISAs work.

    Read More

    Abcam Scientific Support

    Answered on Sep 05 2006

    Immunohistochemistry (Frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Rat mesencephalic brain)
    Specification
    Rat mesencephalic brain
    Fixative
    Paraformaldehyde
    Read More

    Dr. Sophie Pezet

    Verified customer

    投稿 Apr 12 2006

    Question

    I am interested in purchasing the antibody -- Rabbit polyclonal to Tyrosine Hydroxylase (Cat. # 6211). Here I inquire about whether the ab6211 can react with human tissue or not? If not yet tested in your company, could you kindly send me some trial sample, so, I can make it for testing.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 01 2004

    Answer

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soona s any new information is brought to our attention. As far as we are aware, cross reactivity with human has not yet been tested for use with ab6211. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. Please let me know if you require any further assistance.

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    Abcam Scientific Support

    Answered on Jun 01 2004

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