Anti-Tyrosine Hydroxylase 抗体 (ab6211)
Key features and details
- Rabbit polyclonal to Tyrosine Hydroxylase
- Suitable for: IHC-FoFr, WB, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Guinea pig, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Tyrosine Hydroxylase antibody
Tyrosine Hydroxylase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Tyrosine Hydroxylase -
由来種
Rabbit -
特異性
Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme. -
アプリケーション
適用あり: IHC-FoFr, WB, ICC/IF, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Guinea pig, Human
交差が予測される動物種: Chicken, Chimpanzee, Macaque monkey -
免疫原
Synthetic peptide corresponding to Rat Tyrosine Hydroxylase aa 32-47 conjugated to Keyhole Limpet Haemocyanin (KLH) (Glutaraldehyde).
Sequence:PRFIGRRQSLIEDARK
Database link: P04177 -
特記事項
This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
一次抗体 備考
This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab6211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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IHC-FoFr | Use at an assay dependent concentration. | |
WB | 1/100 - 1/500. Predicted molecular weight: 42,53,84 kDa. | |
ICC/IF | 1/1000. | |
IHC-Fr | Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays an important role in the physiology of adrenergic neurons. -
組織特異性
Mainly expressed in the brain and adrenal glands. -
パスウェイ
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
関連疾患
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
配列類似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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参照データベース
- Entrez Gene: 7054 Human
- Entrez Gene: 21823 Mouse
- Entrez Gene: 25085 Rat
- Omim: 191290 Human
- SwissProt: P07101 Human
- SwissProt: P24529 Mouse
- SwissProt: P04177 Rat
- Unigene: 435609 Human
see all -
別名
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
画像
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom
Tyrosine hydroxylase antibody (ab6211) staining rat mesencephalic serotoninergic neurons. Pictures taken using objectives X20 (A) or X20 (B). Note that the staining is observed in the cell body and the processes of these neurons. ab6211 was used at 1/1000 incubated for 2 days at room temperature. Secondary antibody was anti-rabbit Alexa Fluor 488 used at 1/1000 for 2 hours at room temperature. Rat brain tissue (ab29475) was perfusion fixed (paraformaldehyde 4% ) followed by post-fixation overnight in the same fixative, cryoprotected in 20% sucrose and then frozen in OCT. 30µm coronal sections of brain were cutting by cyrostat and immunohistochemistry was performed as the 'free floating' technique.
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ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Anti-Tyrosine Hydroxylase antibody (ab6211) at 1/100 dilution + PC12 cell lysates at 6 µg
Performed under reducing conditions.
Predicted band size: 42,53,84 kDaThree bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa.
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Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)Image courtesy of QBM Cell Sciences, Ottawa, Canadaab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1:100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
データシートおよび資料
参考文献 (41)
ab6211 は 41 報の論文で使用されています。
- Yang SY et al. Glucocerebrosidase activity, cathepsin D and monomeric a-synuclein interactions in a stem cell derived neuronal model of a PD associated GBA1 mutation. Neurobiol Dis 134:104620 (2020). PubMed: 31634558
- Liu AQ et al. Sensory nerve-deficient microenvironment impairs tooth homeostasis by inducing apoptosis of dental pulp stem cells. Cell Prolif 53:e12803 (2020). PubMed: 32246537
- Rosas-Jarquín CJ et al. Chronic consumption of cassava juice induces cellular stress in rat substantia nigra. Iran J Basic Med Sci 23:93-101 (2020). PubMed: 32405352
- Han X et al. Small molecule-driven NLRP3 inflammation inhibition via interplay between ubiquitination and autophagy: implications for Parkinson disease. Autophagy 15:1860-1881 (2019). PubMed: 30966861
- Lohoff FW et al. Deletion of the vesicular monoamine transporter 1 (vmat1/slc18a1) gene affects dopamine signaling. Brain Res N/A:N/A (2019). PubMed: 30685272