Anti-Tyrosine Hydroxylase 抗体 (ab113)
製品の概要
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製品名
Anti-Tyrosine Hydroxylase antibody
Tyrosine Hydroxylase 一次抗体 製品一覧 -
製品の詳細
Sheep polyclonal to Tyrosine Hydroxylase -
由来種
Sheep -
アプリケーション
適用あり: IHC-FrFl, ICC/IF, IHC-Fr, WB, IP, IHC-FoFrmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Mammals
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免疫原
Full length native protein (purified) corresponding to Rat Tyrosine Hydroxylase. SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma (rat)
Database link: P04177 -
ポジティブ･コントロール
- Rat brain normal tissue lysate - total protein (ab29475) can be used as a positive control in WB.
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特記事項
ab113 can be used as a marker for dopaminergic and noradrenergic neurons.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.50
Constituents: PBS, 0.01% BSA, 0.87% Sodium chloride, 50% Glycerol, 0.238% HEPES -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FrFl | 1/1000 - 1/2000. | |
| ICC/IF | 1/1000. | |
| IHC-Fr | 1/1000. | |
| WB | 1/200. Predicted molecular weight: 60 kDa. | |
| IP | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays an important role in the physiology of adrenergic neurons. -
組織特異性
Mainly expressed in the brain and adrenal glands. -
パスウェイ
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
関連疾患
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
配列類似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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参照データベース
- Entrez Gene: 21823 Mouse
- Entrez Gene: 25085 Rat
- SwissProt: P24529 Mouse
- SwissProt: P04177 Rat
- Unigene: 1292 Mouse
- Unigene: 11082 Rat
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別名
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
画像
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Immunohistochemistry - Free Floating - Anti-Tyrosine Hydroxylase antibody (ab113)Image courtesy of Dr Xiaobin Liu by Abreview.ab113 staining Tyrosine Hydroxylase in mouse brain tissue by Immunohistochemistry (Free Floating). Tissue was incubated with ab113 at a 1/1000 dilution for 48 hours at 4°C. The secondary used was a Dylight 594 conjugated donkey anti-sheep IgG (H+L) (ab96941) used at a 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab113)This image is courtesy of an Abreview submitted by Jim Deuchars.Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.
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Immunohistochemistry - Free Floating - Anti-Tyrosine Hydroxylase antibody (ab113)ab113 at 1/2000 dilution staining rat brain cells (ab29475) by immunocyctochemistry. The antibody was incubated with the tissue for 48 hours and then bound antibody was detected using a biotinylated donkey anti-sheep antibody. The image shows the staining of free-floating 50 micron brainstem sections, labelling A5cell groups. The antibody also labels fine varicosities of axons.
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Western blot - Anti-Tyrosine Hydroxylase antibody (ab113)Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k tyrosine hydroxylase protein.
プロトコール
データシートおよび資料
参考文献
ab113 は 35 報の論文で使用されています。
- Xia Y et al. Microglia as modulators of exosomal alpha-synuclein transmission. Cell Death Dis 10:174 (2019). PubMed: 30787269
- Ma K et al. Reduced VMAT2 expression exacerbates the hyposmia in the MPTP model of Parkinson's disease. Biochem Biophys Res Commun 513:306-312 (2019). PubMed: 30954223
- Bülbül M et al. Central neuropeptide-S treatment improves neurofunctions of 6-OHDA-induced Parkinsonian rats. Exp Neurol 317:78-86 (2019). PubMed: 30825442
- Hernández-Pérez OR et al. A Synaptically Connected Hypothalamic Magnocellular Vasopressin-Locus Coeruleus Neuronal Circuit and Its Plasticity in Response to Emotional and Physiological Stress. Front Neurosci 13:196 (2019). PubMed: 30949017
- Kostuk EW et al. Subregional differences in astrocytes underlie selective neurodegeneration or protection in Parkinson's disease models in culture. Glia 67:1542-1557 (2019). PubMed: 31025779
