製品の概要

  • 製品名
    Anti-Tyrosine Hydroxylase antibody
    Tyrosine Hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to Tyrosine Hydroxylase
  • 由来種
    Sheep
  • アプリケーション
    適用あり: IHC-FrFl, ICC/IF, IHC-Fr, WB, IP, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Mammals
  • 免疫原

    Full length native protein (purified) corresponding to Rat Tyrosine Hydroxylase. SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma (rat)
    Database link: P04177

  • ポジティブ・コントロール
    • Rat brain normal tissue lysate - total protein (ab29475) can be used as a positive control in WB.
  • 特記事項

    ab113 can be used as a marker for dopaminergic and noradrenergic neurons.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FrFl 1/1000 - 1/2000.
ICC/IF 1/1000.
IHC-Fr 1/1000.
WB 1/200. Predicted molecular weight: 60 kDa.
IP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Plays an important role in the physiology of adrenergic neurons.
  • 組織特異性
    Mainly expressed in the brain and adrenal glands.
  • パスウェイ
    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • 関連疾患
    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • 配列類似性
    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

画像

  • ab113 staining Tyrosine Hydroxylase in murine brain tissue by Immunohistochemistry (Free Floating).Tissue was incubated with ab113 at a 1/1000 dilution for 48 hours at 4°C. The secondary used was a Dylight 594 conjugated donkey anti-sheep IgG (H+L) (ab96941) used at a 1/500 dilution.

    See Abreview

  • Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.

  • ab113 at 1/2000 dilution staining rat brain cells (ab29475) by immunocyctochemistry. The antibody was incubated with the tissue for 48 hours and then bound antibody was detected using a biotinylated donkey anti-sheep antibody. The image shows the staining of free-floating 50 micron brainstem sections, labelling A5cell groups. The antibody also labels fine varicosities of axons.

    This image is courtesy of an Abreview submitted by Jim Deuchars.

    See Abreview

  • Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k tyrosine hydroxylase protein.

参考文献

This product has been referenced in:
  • Struzyna LA  et al. Tissue engineered nigrostriatal pathway for treatment of Parkinson's disease. J Tissue Eng Regen Med 12:1702-1716 (2018). Read more (PubMed: 29766664) »
  • Chauvet N  et al. Complementary actions of dopamine D2 receptor agonist and anti-vegf therapy on tumoral vessel normalization in a transgenic mouse model. Int J Cancer 140:2150-2161 (2017). Read more (PubMed: 28152577) »
See all 27 Publications for this product

レビューと Q&A

1-7 of 7 Abreviews or Q&A

Answer

Thank you for your email.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab113.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Read More
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain slice)
Specification
brain slice

Dr. Xiaobin Liu

Verified customer

投稿 Jul 18 2011

Question
Answer

Thank you for your patience. I have received confirmation from the originator of the product and it seems that there is no specific data on whether the antibody recognizes all isoforms of histone H1, as we do not currently have access to the antigen. This data was originally determined via ELISA. I am sorry I can't be of more assistance in this occasion but should you require any further information, please do not hesitate to contact me.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (brain)
Specification
brain
Fixative
Paraformaldehyde

Prof. Jim Deuchars

Verified customer

投稿 May 16 2006

Answer

Dear Sir: Both of these antibodies can be used for immunocytochemistry and immunohistochemistry. The difference is that the NB300-108 is sensitive enough to stain fibers in the cerebral cortex. The cost of these two antibodies is $175. We would ship these antibodies, using dry ice ($10) and the delivery charge would be $60. This would be a total of $245 (USD). Delivery would take 2 business days to arrive to you from our establishment. I hope this reply answers all of your questions. Best Regards, Kate Lynott, DSO Novus Biologicals, Inc.

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Answer

The antibody should work for this application. For a reference, see: Lewis, D.A., et al. Four Isoforms of Tyrosine Hydroxylase are Expressed in Human Brain. 54(2): 477-492, 1993.

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Answer

raised against native rat - purified from pheochromocytoma, host species is sheep, can be used to detect all mammalian forms,WB gives a band at ~60 kDa [1:200 dilution (S35-protein A)], can be used in IP (1:10), IH, and IC (1:2000),supplied in 15 ug units at 100 ul, each. For more information, please check the www.novus-biolocals.com webpage (catalog # NB300-110).

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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