製品の概要

  • 製品名
    Anti-Tyrosine Hydroxylase antibody
    Tyrosine Hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Tyrosine Hydroxylase
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-Fr, IHC-P, IHC-FrFl, ICC/IF, IHC-FoFr, WB, IP, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Goat, Cat, Human, Pig
    交差が予測される動物種: Mammals
  • 免疫原

    Full length SDS denatured protein (purified from pheochromocytoma) (Rat).

  • ポジティブ・コントロール
    • IHC-P: Mouse and rat brain tissue. Rat dopaminergic neuronal tissue. ICC/IF: Human neurons. WB: Rat brain normal tissue lysate - total protein (ab29475), Mouse cell lysate (CATH.a neuron). PC-12 whole cell lysate. Rat caudate lysate. IHC-Fr: Mouse brain tissue.
  • 特記事項

    ab112 can be used as a marker for dopaminergic and noradrenergic neurons.

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/750 - 1/1500.
IHC-P 1/750.
IHC-FrFl 1/1000. PubMed: 222726
ICC/IF 1/1000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20357108
WB 1/200. Predicted molecular weight: 60 kDa.
IP Use at an assay dependent concentration.
ICC 1/2000.

ターゲット情報

  • 機能
    Plays an important role in the physiology of adrenergic neurons.
  • 組織特異性
    Mainly expressed in the brain and adrenal glands.
  • パスウェイ
    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • 関連疾患
    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • 配列類似性
    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

画像

  • Effects of hypercholesterolemia on tyrosine hydroxylase (TH)-immunoreactivity in striatum (NCP) and TH-positive nigral (SN) neurons in MPTP-treated mice.

    Representative NCP (A-D) and SN (E-H) photographs from CS, HCD, MPTP and HCD+MPTP (left to right) groups showing TH-immunoreactivity.

    Mice were anesthetized with chloral hydrate (350 mg/kg; i.p.) and perfused intracardially with phosphate buffered saline (PBS, 0.1 M; pH 7.4) followed by 4% w/v PFA in PBS. Brains were removed and kept overnight in the same fixative, transferred to 30% w/v sucrose solution. 20 μm thick coronal sections passing through the NCP and SN were taken using Cryotome in poly-L-lysine coated slides. The sections were rinsed three times with 0.1 M Tris-buffered saline (TBS, 0.1 M; pH 7.4), incubated in 3% H2O2 in TBS, permeabilized with 0.3% Triton X-100, and blocked with 10% donkey serum containing 0.3% Triton X-100. The sections were incubated overnight with primary antibody for TH ab112 (1:700) in TBS, containing 2% donkey serum at 4°C and then incubated with HRP-conjugated secondary antibody (1:1000) in TBS for 1 h at room temperature. Colour development was performed by incubating the sections in DAB-liquid substrate system and then sections were washed, dehydrated, cleared in xylene, mounted in DPX and photographed under bright field illumination using a digital SLR camera.

  • Lateral localization of CB1-R-dense striosome-dendron bouquets and ventral tier SNpc enveloping CB1-R-immunoreactive axons through the substantia nigra (mouse).

    An example of a TH- and CB1-R-positive dendron in the rostral SN is shown in Panel C.

    Coronal vibratome sections (40 μm) or glycerol cryoprotected frozen sliding microtome sections (30 μm) were taken at levels extending from the prefrontal cortex to the ventral mesencephalon, and every 3rd to 6th section, depending on the experiment, was stained and imaged. Sections were washed in PBS-T (0.2% Triton X-100), blocked for 4 hours with 5% BSA in PBST, and incubated in primary antibodies overnight to 48 hours in a cold room on an orbital shaker.

  • ab112 staining Tyrosine Hydroxylase in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/800 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    The image of Caudate putamen shows characteristic nerve fibre positivity

    See Abreview

  • ab112 staining tyrosine hydroxulase in mouse brain tissue.

    Sections were permeabilized with 0.5% Triton X-100 in PBS at room temperature for 10 minutes. Samples were incubated with primary antibody (1:500) overnight at 4C.

  • PFA-fixed, Triton X-100 permeabilized human neurons in cerebral organoid stained for Tyrosine Hydroxylase (green) using ab112 at 1/300 dilution in ICC/IF.

  • All lanes : Anti-Tyrosine Hydroxylase antibody (ab112) at 1/200 dilution

    All lanes : Mouse cell lysate (CATH.a neuron)

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit HRP

    Developed using the ECL technique.

    Predicted band size: 60 kDa


    Exposure time: 50 seconds
  • ab112 at 1/500 staining rat brain tissue (ab29475) sections by IHC-P.

    The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 14 hours. A biotinylated swine anti-rabbit IgG was used as the secondary.

    See Abreview

  • ab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry.

    The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.

    See Abreview

  • Anti-Tyrosine Hydroxylase antibody (ab112) at 1/200 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Predicted band size: 60 kDa

  • Paraffin embedded sections of rat brain tissue were stained for Tyrosine Hydroxylase with ab112 at 1/5000 dilution in immunohistochemical analysis (Panel 1).

    Panel 2 shows an image in which ab112 was replaced with a Rabbit IgG1 isotype control.

  • Anti-Tyrosine Hydroxylase antibody (ab112) at 1/200 dilution + rat caudate lysate at 10 µg

    Predicted band size: 60 kDa

  • ab112, at 1/750, staining tyrosine hydroxylase in mouse brain tissue (ab30151) by Immunohistochemistry (Frozen sections).

    Sections were PFA fixed, permeabilized in 0.3 Triton X-100 prior to blocking in 5% serum for 1 hour at 22°C and then incubated with ab112, for 16 hours at 22°C. Alexa fluor® 555 goat polyclonal to rabbit Ig (ab150078), diluted 1/1000, was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • He K  et al. Near-infrared Intraoperative Imaging of Thoracic Sympathetic Nerves: From Preclinical Study to Clinical Trial. Theranostics 8:304-313 (2018). Read more (PubMed: 29290809) »
  • Jeong GR  et al. Dysregulated phosphorylation of Rab GTPases by LRRK2 induces neurodegeneration. Mol Neurodegener 13:8 (2018). Read more (PubMed: 29439717) »
See all 91 Publications for this product

レビューと Q&A

1-10 of 40 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brainstem)
Antigen retrieval step
None
Permeabilization
No
Specification
brainstem
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Sep 07 2018

Application
Western blot
Sample
Sheep Tissue lysate - whole (Adipose fat)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
Adipose fat
Blocking step
Odyssey Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C

Kanchan Kaushal

Verified customer

投稿 Sep 06 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Jun 29 2018

Application
Western blot
Sample
Rat Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Jun 28 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 May 16 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (medulla oblongata)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization
No
Specification
medulla oblongata
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Apr 03 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus monkey Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Brain
Blocking step
Protein Block (ab64226) as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Jan 10 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Brain
Blocking step
Protein Block (ab64226) as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Dec 21 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Brain
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Nov 13 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (neurons in human cerebral organoid)
Permeabilization
Yes - Triton X-100
Specification
neurons in human cerebral organoid
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 37°C
Fixative
Paraformaldehyde

Radhika Menon

Verified customer

投稿 Jul 12 2017

1-10 of 40 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録