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    tyrosinase-antibody-t311-ab738.pdf

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Anti-Tyrosinase 抗体 [T311] (ab738)

  • Datasheet
Reviews (2)Q&A (4)References (11)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Mouse monoclonal [T311] to Tyrosinase
  • Reacts with: Human
  • Isotype: IgG2a

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human Tyrosinase protein (Tagged) (ab152776)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-Tyrosinase antibody [T311]
    Tyrosinase 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [T311] to Tyrosinase
  • 由来種

    Mouse
  • 特異性

    Studies have shown tyrosinase to be a very specific marker for melanomas, not cross reacting with any other tumors or normal tissues tested Other studies have shown tyrosinase to be a more sensitive marker when compared to HMB-45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB-45. However, both tyrosinase and MART-1 negative staining was seen in those variants without an epidermal component. Unlike HMB-45, neither tyrosinase or MART-1 discriminates between activated or resting melanocytes. In conclusion, tyrosinase appears to be a superior melanoma marker when compared to HMB-45.
  • 種交差性

    交差種: Human
  • 免疫原

    Recombinant full length protein.

  • ポジティブ・コントロール

    • Melanoma
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on [20/07/17]. The following lots are from ascites and are still in stock as of [20/07/17] – [GR278593]. Lot numbers higher than [GR278593] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.099% Sodium azide
    Constituents: 0.9% Proprietary component, 99% Water
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • ポリ/モノ

    モノクローナル
  • クローン名

    T311
  • ミエローマ

    unknown
  • アイソタイプ

    IgG2a
  • 軽鎖の種類

    kappa
  • 研究分野

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Assay kits

    • Tyrosinase Inhibitor Assay Kit (Colorimetric) (ab204715)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human Tyrosinase protein (Tagged) (ab152776)

ターゲット情報

  • 機能

    This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
  • 関連疾患

    Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
    Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.
  • 配列類似性

    Belongs to the tyrosinase family.
  • 細胞内局在

    Melanosome membrane.
  • Target information above from: UniProt accession P14679 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 7299 Human
    • Omim: 606933 Human
    • SwissProt: P14679 Human
    • Unigene: 503555 Human
    • 別名

      • ATN antibody
      • CMM8 antibody
      • LB24 AB antibody
      • LB24-AB antibody
      • Monophenol monooxygenase antibody
      • OCA1 antibody
      • OCA1A antibody
      • OCAIA antibody
      • Oculocutaneous albinism IA antibody
      • SHEP3 antibody
      • SK29 AB antibody
      • SK29-AB antibody
      • Tumor rejection antigen AB antibody
      • TYR antibody
      • TYRO_HUMAN antibody
      • tyrosinase (oculocutaneous albinism IA) antibody
      • Tyrosinase antibody
      see all

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
  • 参考文献 (11)

    ab738 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab738 は 11 報の論文で使用されています。

    • Du R & Lei T Effects of autologous platelet-rich plasma injections on facial skin rejuvenation. Exp Ther Med 19:3024-3030 (2020). PubMed: 32256789
    • Kawakami T  et al. Approach for the Derivation of Melanocytes from Induced Pluripotent Stem Cells. J Invest Dermatol 138:150-158 (2018). PubMed: 28887108
    • Margaryan NV  et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci 17:418 (2016). WB . PubMed: 27011171
    • Allouche J  et al. In vitro modeling of hyperpigmentation associated to neurofibromatosis type 1 using melanocytes derived from human embryonic stem cells. Proc Natl Acad Sci U S A 112:9034-9 (2015). Human . PubMed: 26150484
    • Loubéry S  et al. Myosin VI regulates actin dynamics and melanosome biogenesis. Traffic 13:665-80 (2012). PubMed: 22321127
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Question

    What is the concentration of lot GR33380?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 17 2012

    Answer

    Thank you for contacting us.

    The IgG concentration of lot GR33380 is 66.7ug/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jul 17 2012

    Western blot abreview for Anti-Tyrosinase antibody [T311 ]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A375 - melanoma cell line)
    Loading amount
    10000 cells
    Specification
    A375 - melanoma cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 31 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosinase antibody [T311 ]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris - EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with Tween
    Read More

    Mr. Rudolf Jung

    Verified customer

    投稿 Sep 06 2011

    Question

    I would like to enquire about recombinant controls for antibodies I recently purchased from you. Are there any recombinant controls or alternative controls for western blotting for the following antibodies; ab738 ab22601 ab1872 ab23798

    Read More

    Abcam community

    Verified customer

    Asked on Nov 15 2005

    Answer

    Thank you for your email. Below I have listed some suggestions for controls to use with these antibodies. If you have any additional questions, please contact Ab23798 - We do sell recombinant Il-1 beta, it is catalog numbers ab9617, ab9723, and ab9788. Also, the antibody clearly stains IL1b positive cells in liver, spleen, pituitary and brain of endotoxin treated rats, so those tissues can be used as a positive control as well. Ab1872 - Placenta is recommended as a positive control. Also, caspase 1 is expressed in larger amounts in spleen and lung. Also found in liver, heart, skeletal muscle, kidney and testis. No expression in the brain. Ab738 - Melanoma is recommended as a positive control. Ab22601 - I couldn't find much information except for this: Msx2 mRNA is found expressed at higher levels in immature tumors, including a yolk sac tumor, seminoma, and chroiocarcinoma, and also in various tumors of epithelial origin. The gene expression is frequently activated in carcinoma derived cell lines.

    Read More

    Abcam Scientific Support

    Answered on Nov 15 2005

    Question

    Has the epitope been mapped?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 07 2005

    Answer

    Thank you for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

    Read More

    Abcam Scientific Support

    Answered on Mar 11 2005

    Question

    Your spec-sheet for tyrosinase antibody (ab738) does not have any information for western blotting. I would like to know at what size the band comes out, and how much I need to dilute the antibody for western blotting. And what kind of western protocol would you recommend me using, milk based? BSA-based? Or other ? I would appreciate your prompt response.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 24 2005

    Answer

    Thank you for your enquiry regarding ab738 and its use in WB. It has been reported in the literature that this clone T311 works in western however we have not tested it ourselves, I apologise for the inconvenience. We would recommend doing a preliminary experiment to determine the optimal dilution (a range of dilutions should be tried e.g 1:50-1:500) and we usually recommend to try BSA 5% in TBST (1hr, RT or 4C) for blocking. Please do not hesitate to contact us if you need any more advice or information or have any problems with your experiment,

    Read More

    Abcam Scientific Support

    Answered on Feb 27 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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