製品の概要
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製品名Anti-Tyrosinase antibody [T311]
Tyrosinase 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [T311] to Tyrosinase
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由来種Mouse
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特異性Studies have shown tyrosinase to be a very specific marker for melanomas, not cross reacting with any other tumors or normal tissues tested Other studies have shown tyrosinase to be a more sensitive marker when compared to HMB-45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB-45. However, both tyrosinase and MART-1 negative staining was seen in those variants without an epidermal component. Unlike HMB-45, neither tyrosinase or MART-1 discriminates between activated or resting melanocytes. In conclusion, tyrosinase appears to be a superior melanoma marker when compared to HMB-45.
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アプリケーション適用あり: ICC/IF, IHC-P, IHC-Fr, WBmore details
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種交差性交差種: Human
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免疫原
Recombinant full length protein.
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ポジティブ･コントロール
- Melanoma
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特記事項
This product was changed from ascites to tissue culture supernatant on [20/07/17]. The following lots are from ascites and are still in stock as of [20/07/17] – [GR278593]. Lot numbers higher than [GR278593] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.099% Sodium azide
Constituents: 0.9% Proprietary component, 99% Water -
精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名T311
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ミエローマunknown
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アイソタイプIgG2a
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軽鎖の種類kappa
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研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Unmasking reagent
アプリケーション
Our Abpromise guarantee covers the use of ab738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. |
ターゲット情報
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機能This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
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関連疾患Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C. -
配列類似性Belongs to the tyrosinase family.
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細胞内局在Melanosome membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 7299 Human
- Omim: 606933 Human
- SwissProt: P14679 Human
- Unigene: 503555 Human
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別名
- ATN antibody
- CMM8 antibody
- LB24 AB antibody
see all
画像
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![Western blot - Anti-Tyrosinase antibody [T311] (ab738)](https://www.abcam.co.jp/ps/products/0/ab738/Images/Tyrosinase-Primary-antibodies-ab738-15.jpg)
Western blot - Anti-Tyrosinase antibody [T311] (ab738)This image is courtesy of an anonymous AbreviewAnti-Tyrosinase antibody [T311] (ab738) at 1/250 dilution + Human A375 whole cell lysate at 10000 cells
Secondary
HRP-conjugated Horse anti-mouse IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 65 kDa (why is the actual band size different from the predicted?)
Exposure time: 10 minutes
Blocked with 5% Milk for 1 hour at 20°C -
![Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody [T311] (ab738)](https://www.abcam.co.jp/ps/products/0/ab738/Images/Tyrosinase-Primary-antibodies-ab738-16.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody [T311] (ab738)Image from Ginger RS et al, J Biol Chem. 2008 Feb 29;283(9):5486-95. Epub 2007 Dec 28, Fig 3. DOI 10.1074/jbc.M707521200 February 29, 2008 The Journal of Biological Chemistry, 283, 5486-5495.ab738 staining Tyrosinase in human neonatal foreskin (NHM) cells by Immunocytochemistry/ Immunofluorescence.
NHM grown on coverslips were fixed and probed with anti-NCKX5-(I) (green) and ab738 (red). Merged images are shown.
プロトコール
参考文献
This product has been referenced in:
- Margaryan NV et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci 17:418 (2016). WB . Read more (PubMed: 27011171) »
- Allouche J et al. In vitro modeling of hyperpigmentation associated to neurofibromatosis type 1 using melanocytes derived from human embryonic stem cells. Proc Natl Acad Sci U S A 112:9034-9 (2015). Human . Read more (PubMed: 26150484) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"