Anti-Tyrosinase 抗体 [T311] (ab738)
Key features and details
- Mouse monoclonal [T311] to Tyrosinase
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Tyrosinase antibody [T311]
Tyrosinase 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [T311] to Tyrosinase -
由来種
Mouse -
特異性
Studies have shown tyrosinase to be a very specific marker for melanomas, not cross reacting with any other tumors or normal tissues tested Other studies have shown tyrosinase to be a more sensitive marker when compared to HMB-45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB-45. However, both tyrosinase and MART-1 negative staining was seen in those variants without an epidermal component. Unlike HMB-45, neither tyrosinase or MART-1 discriminates between activated or resting melanocytes. In conclusion, tyrosinase appears to be a superior melanoma marker when compared to HMB-45. -
種交差性
交差種: Human -
免疫原
Recombinant full length protein.
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ポジティブ･コントロール
- Melanoma
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特記事項
This product was changed from ascites to tissue culture supernatant on [20/07/17]. The following lots are from ascites and are still in stock as of [20/07/17] – [GR278593]. Lot numbers higher than [GR278593] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.099% Sodium azide
Constituents: 0.9% Proprietary component, 99% Water -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
T311 -
ミエローマ
unknown -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
ターゲット情報
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機能
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. -
関連疾患
Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C. -
配列類似性
Belongs to the tyrosinase family. -
細胞内局在
Melanosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7299 Human
- Omim: 606933 Human
- SwissProt: P14679 Human
- Unigene: 503555 Human
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別名
- ATN antibody
- CMM8 antibody
- LB24 AB antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab738 は 11 報の論文で使用されています。
- Du R & Lei T Effects of autologous platelet-rich plasma injections on facial skin rejuvenation. Exp Ther Med 19:3024-3030 (2020). PubMed: 32256789
- Kawakami T et al. Approach for the Derivation of Melanocytes from Induced Pluripotent Stem Cells. J Invest Dermatol 138:150-158 (2018). PubMed: 28887108
- Margaryan NV et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci 17:418 (2016). WB . PubMed: 27011171
- Allouche J et al. In vitro modeling of hyperpigmentation associated to neurofibromatosis type 1 using melanocytes derived from human embryonic stem cells. Proc Natl Acad Sci U S A 112:9034-9 (2015). Human . PubMed: 26150484
- Loubéry S et al. Myosin VI regulates actin dynamics and melanosome biogenesis. Traffic 13:665-80 (2012). PubMed: 22321127