Anti-Tyrosinase 抗体 [T311] (ab738)

Reviews (2)Q&A (4)References (8)

製品の概要

  • 製品名

    Anti-Tyrosinase antibody [T311]
    Tyrosinase 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [T311] to Tyrosinase

  • 由来種

    Mouse

  • 特異性

    Studies have shown tyrosinase to be a very specific marker for melanomas, not cross reacting with any other tumors or normal tissues tested Other studies have shown tyrosinase to be a more sensitive marker when compared to HMB-45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB-45. However, both tyrosinase and MART-1 negative staining was seen in those variants without an epidermal component. Unlike HMB-45, neither tyrosinase or MART-1 discriminates between activated or resting melanocytes. In conclusion, tyrosinase appears to be a superior melanoma marker when compared to HMB-45.

  • アプリケーション

    適用あり: ICC/IF, IHC-P, IHC-Fr, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein.

  • ポジティブ･コントロール

    • Melanoma

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on [20/07/17]. The following lots are from ascites and are still in stock as of [20/07/17] – [GR278593]. Lot numbers higher than [GR278593] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

  • 機能

    This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.

  • 関連疾患

    Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
    Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.

  • 配列類似性

    Belongs to the tyrosinase family.

  • 細胞内局在

    Melanosome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ATN antibody
      • CMM8 antibody
      • LB24 AB antibody
      • LB24-AB antibody
      • Monophenol monooxygenase antibody
      • OCA1 antibody
      • OCA1A antibody
      • OCAIA antibody
      • Oculocutaneous albinism IA antibody
      • SHEP3 antibody
      • SK29 AB antibody
      • SK29-AB antibody
      • Tumor rejection antigen AB antibody
      • TYR antibody
      • TYRO_HUMAN antibody
      • tyrosinase (oculocutaneous albinism IA) antibody
      • Tyrosinase antibody
      see all

    画像

    • Western blot - Anti-Tyrosinase antibody [T311] (ab738)
      Western blot - Anti-Tyrosinase antibody [T311] (ab738)This image is courtesy of an anonymous Abreview
      Anti-Tyrosinase antibody [T311] (ab738) at 1/250 dilution + Human A375 whole cell lysate at 10000 cells

      Secondary
      HRP-conjugated Horse anti-mouse IgG polyclonal at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 65 kDa
      why is the actual band size different from the predicted?


      Exposure time: 10 minutes


      Blocked with 5% Milk for 1 hour at 20°C

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody [T311] (ab738)
      Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody [T311] (ab738)Image from Ginger RS et al, J Biol Chem. 2008 Feb 29;283(9):5486-95. Epub 2007 Dec 28, Fig 3. DOI 10.1074/jbc.M707521200 February 29, 2008 The Journal of Biological Chemistry, 283, 5486-5495.
      ab738 staining Tyrosinase in human neonatal foreskin (NHM) cells by Immunocytochemistry/ Immunofluorescence.
      NHM grown on coverslips were fixed and probed with anti-NCKX5-(I) (green) and ab738 (red). Merged images are shown.

    参考文献

    This product has been referenced in:

    • Margaryan NV  et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci 17:418 (2016). WB . Read more (PubMed: 27011171) »
    • Allouche J  et al. In vitro modeling of hyperpigmentation associated to neurofibromatosis type 1 using melanocytes derived from human embryonic stem cells. Proc Natl Acad Sci U S A 112:9034-9 (2015). Human . Read more (PubMed: 26150484) »
    See all 8 Publications for this product

    ab738 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-6 of 6 Abreviews or Q&A

    Question

    What is the concentration of lot GR33380?

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    Answer

    Thank you for contacting us.

    The IgG concentration of lot GR33380 is 66.7ug/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Western blot abreview for Anti-Tyrosinase antibody [T311 ]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A375 - melanoma cell line)
    Loading amount
    10000 cells
    Specification
    A375 - melanoma cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 31 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosinase antibody [T311 ]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris - EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with Tween
    Read More

    Mr. Rudolf Jung

    Verified customer

    投稿 Sep 06 2011

    Question

    I would like to enquire about recombinant controls for antibodies I recently purchased from you. Are there any recombinant controls or alternative controls for western blotting for the following antibodies; ab738 ab22601 ab1872 ab23798

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    Answer

    Thank you for your email. Below I have listed some suggestions for controls to use with these antibodies. If you have any additional questions, please contact Ab23798 - We do sell recombinant Il-1 beta, it is catalog numbers ab9617, ab9723, and ab9788. Also, the antibody clearly stains IL1b positive cells in liver, spleen, pituitary and brain of endotoxin treated rats, so those tissues can be used as a positive control as well. Ab1872 - Placenta is recommended as a positive control. Also, caspase 1 is expressed in larger amounts in spleen and lung. Also found in liver, heart, skeletal muscle, kidney and testis. No expression in the brain. Ab738 - Melanoma is recommended as a positive control. Ab22601 - I couldn't find much information except for this: Msx2 mRNA is found expressed at higher levels in immature tumors, including a yolk sac tumor, seminoma, and chroiocarcinoma, and also in various tumors of epithelial origin. The gene expression is frequently activated in carcinoma derived cell lines.

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    Question

    Has the epitope been mapped?

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    Answer

    Thank you for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

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    Question

    Your spec-sheet for tyrosinase antibody (ab738) does not have any information for western blotting. I would like to know at what size the band comes out, and how much I need to dilute the antibody for western blotting. And what kind of western protocol would you recommend me using, milk based? BSA-based? Or other ? I would appreciate your prompt response.

    Read More
    Answer

    Thank you for your enquiry regarding ab738 and its use in WB. It has been reported in the literature that this clone T311 works in western however we have not tested it ourselves, I apologise for the inconvenience. We would recommend doing a preliminary experiment to determine the optimal dilution (a range of dilutions should be tried e.g 1:50-1:500) and we usually recommend to try BSA 5% in TBST (1hr, RT or 4C) for blocking. Please do not hesitate to contact us if you need any more advice or information or have any problems with your experiment,

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