Anti-TXNDC5 抗体 (ab10292)
Key features and details
- Rabbit polyclonal to TXNDC5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TXNDC5 antibody
TXNDC5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TXNDC5 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Mouse TXNDC5 aa 50 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q91W90 -
ポジティブ･コントロール
- WB: HepG2, HeLa and HEK293 whole cell lysate.
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特記事項
ERp19 and ERp46 are two newly discovered ER luminal proteins, related to protein disulphide isomerase. Western and Northern blot analyses have revealed that both ERp19 and ERp46 and their respective mRNAs are highly expressed in the liver as compared with other tissues. Both proteins are enriched in purified liver ER vesicles and were localized specifically to the ER in McA-RH7777 hepatocytes. See Knoblach et al. for details.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
ERp19 and ERp46 are two newly discovered ER luminal proteins, related to protein disulphide isomerase. Western and Northern blot analyses have revealed that both ERp19 and ERp46 and their respective mRNAs are highly expressed in the liver as compared with other tissues. Both proteins are enriched in purified liver ER vesicles and were localized specifically to the ER in McA-RH7777 hepatocytes. See Knoblach et al. for details. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10292の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
ターゲット情報
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機能
Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast. -
配列類似性
Belongs to the protein disulfide isomerase family.
Contains 3 thioredoxin domains. -
細胞内局在
Endoplasmic reticulum lumen. - Information by UniProt
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参照データベース
- Entrez Gene: 81567 Human
- SwissProt: Q8NBS9 Human
- Unigene: 150837 Human
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別名
- EndoPDI antibody
- Endoplasmic reticulum protein ERp46 antibody
- Endoplasmic reticulum resident protein 46 antibody
see all
画像
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Western blot - Anti-TXNDC5 antibody (ab10292)All lanes : Anti-TXNDC5 antibody (ab10292) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 60 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab10292 は 2 報の論文で使用されています。
- Alberti A et al. ERp46 is reduced by high glucose and regulates insulin content in pancreatic beta-cells. Am J Physiol Endocrinol Metab 297:E812-21 (2009). WB, ICC/IF ; Mouse . PubMed: 19622788
- Knoblach B et al. ERp19 and ERp46, New Members of the Thioredoxin Family of Endoplasmic Reticulum Proteins. Mol Cell Proteomics 2:1104-19 (2003). PubMed: 12930873
