Name: Anti-TWEAKR/FN14 antibody [EPR3179]
Brand: RabMAb
SKU: ab109365
Rating: 5 (3 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3179] to TWEAKR/FN14
Suitable for: WB, IP, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-TWEAKR/FN14 antibody [EPR3179]
TWEAKR/FN14 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR3179] to TWEAKR/FN14
由来種

Rabbit
特異性

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
アプリケーション

適用あり: WB, IP, IHC-Pmore details
適用なし: ICC
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- HT-29, NIH/3T3, L6, C2C12, HUVEC and HT-1080 cell lysates; Human uterus tissue, human bladder carcinoma and human cardiac muscle.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR3179
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)
- Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab109365の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IP		1/40. For unpurified use at 1/10.
IHC-P	(3)	1/50 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IP 1/40. For unpurified use at 1/10.
IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

追加情報

Is unsuitable for ICC.

機能

Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
組織特異性

Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
配列類似性

Contains 1 TNFR-Cys repeat.
細胞内局在

Membrane.
Target information above from: UniProt accession Q9NP84 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 51330 Human
- Entrez Gene: 27279 Mouse
- Entrez Gene: 302965 Rat
- Omim: 605914 Human
- SwissProt: Q9NP84 Human
- SwissProt: Q9CR75 Mouse
- Unigene: 355899 Human
- Unigene: 28518 Mouse
別名
- CD 266 antibody
- CD266 antibody
- CD266 antigen antibody
- FGF inducible 14 antibody
- FGF-inducible 14 antibody
- Fibroblast growth factor inducible immediate early response protein 14 antibody
- Fibroblast growth factor-inducible immediate-early response protein 14 antibody
- FN 14 antibody
- FN14 antibody
- TNFRSF 12A antibody
- TNFRSF12A antibody
- TNR12_HUMAN antibody
- Tumor necrosis factor receptor superfamily member 12A antibody
- TWEAK R antibody
- Tweak receptor antibody
- Tweak-receptor antibody
- TweakR antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

ab109365, at 1/50 dilution, staining TWEAKR/FN14 in Human uterus tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

Immunohistochemical analysis of paraffin-embedded human bladder carcinoma sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

Lane 1 : HUVEC (human umbilical vein/vascular endothelium) whole cell lysate
Lane 2 : HT-1080 (human fibrosarcoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 14 kDa
Observed band size: 14-17 kDa why is the actual band size different from the predicted?

Blocking/Diluting buffer 5% NFDM /TBST
Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
Lane 2 : L6 (rat skeletal muscle) whole cell lysate
Lane 3 : C2C12 (mouse muscle) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 14 kDa
Observed band size: 14-17 kDa why is the actual band size different from the predicted?

Blocking/Diluting buffer 5% NFDM /TBST
Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

ab109365 at 1/40 immunoprecipitating TWEAKR/FN14 in HUVEC (human cervix adenocarcinoma) whole cell lysate observed at 14 – 17 KDa (lanes 1 and 2).

Lane 1 (input): HUVEC whole cell lysate 10ug

Lane 2 (+): ab109365 + HUVEC whole cell lysate.

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109365 in HUVEC whole cell lysate

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

Lane 1 : HT-29 cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : HT-1080 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 14 kDa
Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

Immunoprecipitation protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

ab109365 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109365 は 16 報の論文で使用されています。

Xu J et al. Ubiquitously specific protease 4 inhibitor-Vialinin A attenuates inflammation and fibrosis in S100-induced hepatitis mice through Rheb/mTOR signalling. J Cell Mol Med 25:1140-1150 (2021). PubMed: 33295107
Yao Y et al. Prognostic value of novel immune-related genomic biomarkers identified in head and neck squamous cell carcinoma. J Immunother Cancer 8:N/A (2020). PubMed: 32719094
Wang X et al. Tumor Necrosis Factor (TNF) Receptor Expression Determines Keratinocyte Fate upon Stimulation with TNF-Like Weak Inducer of Apoptosis. Mediators Inflamm 2019:2945083 (2019). PubMed: 31885495
Xiao G et al. Ginkgo Flavonol Glycosides or Ginkgolides Tend to Differentially Protect Myocardial or Cerebral Ischemia-Reperfusion Injury via Regulation of TWEAK-Fn14 Signaling in Heart and Brain. Front Pharmacol 10:735 (2019). PubMed: 31333457
Méndez-Barbero N et al. A major role of TWEAK/Fn14 axis as a therapeutic target for post-angioplasty restenosis. EBioMedicine 46:274-289 (2019). PubMed: 31395500

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (skin tumor)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH6

Permeabilization

Specification

skin tumor

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

10% normal buffered formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 16 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (kidney)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6

Permeabilization

Specification

kidney

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

10% buffered normal formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 16 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (bone)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH6

Permeabilization

Specification

bone

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

10% normal buffered formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 16 2016

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

参考文献 (16)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TWEAKR/FN14 antibody [EPR3179]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TWEAKR/FN14 antibody [EPR3179]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TWEAKR/FN14 antibody [EPR3179]