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Anti-TVA 抗体 [PICA187E] (ab271292)

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Western blot - Anti-TVA antibody [PICA187E] (ab271292)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] (ab271292)
  • Immunocytochemistry - Anti-TVA antibody [PICA187E] (ab271292)
  • Flow Cytometry - Anti-TVA antibody [PICA187E] (ab271292)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] (ab271292)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] (ab271292)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] (ab271292)
  • Anti-TVA antibody [PICA187E] (ab271292)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [PICA187E] to TVA
  • Suitable for: WB, IHC-P, ICC, Flow Cyt
  • Reacts with: Bird

製品の概要

  • 製品名

    Anti-TVA antibody [PICA187E]
    TVA 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [PICA187E] to TVA

  • 由来種

    Rat

  • アプリケーション

    適用あり: WB, IHC-P, ICC, Flow Cytmore details

  • 種交差性

    交差種: Bird

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK-293T transfected with TVA expression vector containing a myc-His-tag®, whole cell lysate. IHC-P: HEK-293T transfected with a TVA construct. Flow Cyt: HEK-293T transfected with myc tagged TVA construct cells. ICC: HEK-293T transfected with myc tagged TVA construct cells.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    PICA187E

  • アイソタイプ

    IgG2b

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
1/5000.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC
1/50.
Flow Cyt
1/1000.
特記事項
WB
1/5000.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC
1/50.
Flow Cyt
1/1000.

ターゲット情報

  • 別名

    • ALV A antibody
    • Avian retroviral receptor for subgroup A antibody

画像

  • Western blot - Anti-TVA antibody [PICA187E] (ab271292)
    Western blot - Anti-TVA antibody [PICA187E] (ab271292)
    All lanes : Anti-TVA antibody [PICA187E] (ab271292) at 1/5000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with TVA expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/50000 dilution

    Observed band size: 25-37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] (ab271292)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] (ab271292)

    Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T transfected with a TVA construct and (Panel B) HEK-293T transfected with empty plasmid, labeling TVA with ab271292 at 1/100 (10.98 µg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on (Panel A) HEK-293T transfected with a TVA construct, no staining on (Panel B) HEK-293T transfected with empty plasmid. The section was incubated with ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry - Anti-TVA antibody [PICA187E] (ab271292)
    Immunocytochemistry - Anti-TVA antibody [PICA187E] (ab271292)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney) cells transfected with myc-tagged TVA expression vector labelling TVA with ab271292 at 1/50 (21.96 µg/ml) dilution, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with myc-tagged TVA expression vector. Myc-Tag mouse mAb (Alexa Fluor® 647) was used as a counterstain at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Used PBS instead of ab271292, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

     

  • Flow Cytometry - Anti-TVA antibody [PICA187E] (ab271292)
    Flow Cytometry - Anti-TVA antibody [PICA187E] (ab271292)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEH-293T (human embryonic kidney epithelial cell) (transfected with myc tagged TVA construct) cells labelling TVA with ab271292 at 1/1000 dilution (0.1µg) (Right panel) compared with a rat monoclonal IgG isotype control (Left panel). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) was used as the secondary antibody at a 1/2000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] (ab271292)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] (ab271292)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TVA with ab271292 at 1/100 (10.98 µg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Negative control: No staining on human cerebrum. The section was incubated with ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] (ab271292)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] (ab271292)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TVA with ab271292 at 1/100 (10.98 µg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Negative control: No staining on mouse cerebrum. The section was incubated with ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] (ab271292)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] (ab271292)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TVA with abab271292 at 1/100 (10.98 µg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Negative control: No staining on rat cerebrum. The section was incubated with ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-TVA antibody [PICA187E] (ab271292)
    Anti-TVA antibody [PICA187E] (ab271292)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab271292 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab271292 は論文での使用が確認できていません。

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