Application
Western blot
Sample
Leishmania Cell lysate - whole cell (Leishmania)
Loading amount
10 µg
Specification
Leishmania
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: BSA 5%
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rat antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: ~50 kDa
Additional data
Additional Notes
1 L mexicana 2 L major 3 L donovani 4 L infantum 5 L amazonensis 6 L tarentolae
A non specific band around 60kDa might appear after longer exposure, or lower dilution
A non specific band around 60kDa might appear after longer exposure, or lower dilution
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 17 2011