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    tubulin-antibody-yol134-microtubule-marker-ab6161.pdf

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Anti-Tubulin 抗体 [YOL1/34] - Microtubule Marker (ab6161)

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  • SDS
Reviews (21)Q&A (1)References (129)

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Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Flow Cytometry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

Key features and details

  • Rat monoclonal [YOL1/34] to Tubulin - Microtubule Marker
  • Suitable for: WB, ICC, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 HRP

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human AK5 protein (ab113593)
一次抗体
Product image
Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866)
二次抗体
Product image
Goat Anti-Rat IgG H&L (HRP) (ab97057)

関連製品

製品の概要

  • 製品名

    Anti-Tubulin antibody [YOL1/34] - Microtubule Marker
    Tubulin 一次抗体 製品一覧
  • 製品の詳細

    Rat monoclonal [YOL1/34] to Tubulin - Microtubule Marker
  • 由来種

    Rat
  • アプリケーション

    適用あり: WB, ICC, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Dog, Saccharomyces cerevisiae, Plants, Schizosaccharomyces pombe, a wide range of other species, Mammals, Alligator
  • 免疫原

    Full length native protein (purified) corresponding to Saccharomyces cerevisiae Tubulin.

  • エピトープ

    ab6161 binds to an epitope between amino acids 414 and 422 of alpha tubulin.
  • ポジティブ・コントロール

    • WB: HeLa and NIH3T3 whole cell lysates and rat brain tissue lysate. Flow Cyt: methanol fixed/tween permeabilised HeLa cells. ICC: HeLa, NIH/3T3 and human macrophage cells.
  • 特記事項

    We can conjugate this antibody to FITC for you (please see ab150252 for details). This antibody can be used as a Western blotting loading control (Kops et al.) and as a Microtubule Marker.

    Has been used for the selection of specific recombinant antibodies engineered to incorporate its epitope. It is also useful for studying the function of microtubules.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 精製度

    Protein G purified
  • 一次抗体 備考

    Has been used for the selection of specific recombinant antibodies engineered to incorporate its epitope. It is also useful for studying the function of microtubules.
  • ポリ/モノ

    モノクローナル
  • クローン名

    YOL1/34
  • アイソタイプ

    IgG2a
  • 研究分野

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker

関連製品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195883)
    • Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)
    • HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab196583)
    • Anti-Tubulin antibody [YOL1/34] - BSA and Azide free (ab264065)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Related Products

    • Anisomycin, Protein synthesis inhibitor (ab120495)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6161の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (10)
Use a concentration of 1 µg/ml.
ICC
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

特記事項
WB
Use a concentration of 1 µg/ml.
ICC
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 配列類似性

    Belongs to the tubulin family.
  • 翻訳後修飾

    Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 細胞内局在

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68363 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 10376 Human
    • Entrez Gene: 203068 Human
    • Entrez Gene: 7280 Human
    • Entrez Gene: 22143 Mouse
    • Entrez Gene: 22151 Mouse
    • Entrez Gene: 22153 Mouse
    • Entrez Gene: 500929 Rat
    • Omim: 191130 Human
    • Omim: 602530 Human
    • Omim: 615101 Human
    • SwissProt: P07437 Human
    • SwissProt: P68363 Human
    • SwissProt: Q13885 Human
    • SwissProt: P05213 Mouse
    • SwissProt: Q7TMM9 Mouse
    • SwissProt: Q9D6F9 Mouse
    • SwissProt: Q6P9V9 Rat
    • Unigene: 524390 Human
    • Unigene: 392113 Mouse
    • Unigene: 99661 Rat
    see all
  • 別名

    • Tubulin beta 2b antibody
    • Alpha tubulin antibody
    • Alpha-tubulin ubiquitous antibody
    • beta Ib tubulin antibody
    • CDCBM5 antibody
    • CDCBM6 antibody
    • fd02b12 antibody
    • K ALPHA 1 antibody
    • M40 antibody
    • OK/SW-cl.56 antibody
    • TBA1B_HUMAN antibody
    • TUBA1B antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin alpha 1b antibody
    • Tubulin alpha-1B chain antibody
    • Tubulin alpha-ubiquitous chain antibody
    • Tubulin beta 1b antibody
    • Tubulin beta 2A antibody
    • tubulin beta 2A class IIa antibody
    • Tubulin beta antibody
    • tubulin beta chain antibody
    • tubulin beta class I antibody
    • tubulin beta-1 chain antibody
    • tubulin beta-2A chain antibody
    • tubulin beta-5 chain antibody
    • Tubulin K-alpha-1 antibody
    • tubulin, alpha, ubiquitous antibody
    • tubulin, beta 2A class IIa antibody
    • tubulin, beta polypeptide 2 antibody
    • tubulin, beta polypeptide antibody
    • tubulin, beta, class IIA antibody
    • wu:fd02b12 antibody
    • zgc:55461 antibody
    see all

画像

  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

    ab6161 staining Tubulin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6161 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

    Western blot against tubulin with ab6161 at 1/3000.  Secondary Rabbit anti-Rat IgG HRP (ab6734)was used at 1/2000.  Exposure time: 2mins.

    Lane 1: 20µg/lane HeLa (Human) whole cell lysates (ab7898).

    Lane 2: 20µg/lane 3T3 (Mouse) whole cell lysate (ab7901).

    Lane 3: 20µg/lane Rat brain tissue lysate (ab7942).

  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

    Confocal image of  21 day in vitro rat hippocampal neurons, stained with rat monoclonal antibody to Tubulin - Microtubule Marker (ab6161) in green at 1/500 and Microtubule Associated protein 2 in blue.

    This picture was kindly supplied as part of the review submitted by Dr Jonathon Burman.

  • Flow Cytometry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Flow Cytometry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Overlay histogram showing HeLa cells stained with ab6161 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6161, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

    Cultured human macrophages were used with ab6161 at 1/1000 for immunofluorescence. Cells were fixed with cold 2% formaldehyde for 20mins.

    Green staining is Alexa 568, Blue staining is DAPI stain.

    This cell represents a young macrophage, the staining patterns varied as the cells aged in culture.

  • Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1/2000 dilution

    All lanes : Yeast (Saccharomyces cerevisiae) whole cell extract prepared by bead-beating

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rat antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds

    See Abreview

  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

    ICC/IF image of ab6161 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab6161, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.

  • Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg

    Secondary
    Rabbit polyclonal to Rat IgG - H&L (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 54 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes
  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)This image is courtesy of an anonymous Abreview

    ab6161 staining mouse NIH 3T3 fibroblast cells by ICC/IF. Cells were PFA fixed  and permeabilized in 0.2% Triton X-100 prior to blocking in 5% BSA for 45 minutes at RT.  The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour.  An Alexa Fluor® 568 conjugated goat anti-rat antibody, diluted 1/3000, was used as the secondary.

     

    See Abreview

  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    ab6161 staining tubulin HeLa cells treated with anisomycin (ab120495), by ICC/IF. Increase in tubulin expression correlates with increased concentration of anisomycin as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120495 (anisomycin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6161 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (129)

ab6161 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6161 は 129 報の論文で使用されています。

  • Brunet T  et al. A flagellate-to-amoeboid switch in the closest living relatives of animals. Elife 10:N/A (2021). PubMed: 33448265
  • Zhou X  et al. Cross-compartment signal propagation in the mitotic exit network. Elife 10:N/A (2021). PubMed: 33481703
  • Manica G  et al. EDEM3 Domains Cooperate to Perform Its Overall Cell Functioning. Int J Mol Sci 22:N/A (2021). PubMed: 33671632
  • Messal HA  et al. Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH. Nat Protoc 16:239-262 (2021). PubMed: 33247285
  • Simón Serrano S  et al. Novel Cyclophilin Inhibitor Decreases Cell Proliferation and Tumor Growth in Models of Hepatocellular Carcinoma. Cancers (Basel) 13:N/A (2021). PubMed: 34207224
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 22 Abreviews or Q&A

Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 09 2019

Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 09 2019

Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Monkey Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 09 2019

Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 09 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH-3T3)
Permeabilization
Yes - 0.2% Triton X-100
Specification
NIH-3T3
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Spencer Goodman

Verified customer

投稿 May 23 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (Embryo)
Permeabilization
Yes - Methanol
Specification
Embryo
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Oct 20 2016

Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (BY4741)
Gel Running Conditions
Reduced Denaturing (4%-15%)
Loading amount
10 µg
Specification
BY4741
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Dec 10 2015

Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Leishmania Cell lysate - whole cell (Leishmania)
Loading amount
10 µg
Specification
Leishmania
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Nov 17 2011

Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (Non-small cell lung carcinoma)
Loading amount
10 µg
Specification
Non-small cell lung carcinoma
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Dec 01 2010

Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Candida albicans Cell lysate - whole cell (Candida Albicans Whole Cell Prep)
Gel Running Conditions
Non-reduced Denaturing (10% SDS PAGE)
Loading amount
5e+007 cells
Specification
Candida Albicans Whole Cell Prep
Blocking step
LI-COR Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Oct 07 2009

1-10 of 22 Abreviews or Q&A

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