Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (8)
Sample
Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells)
Specification
Chinese Hamster Ovary cells
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk TBS-Tween
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/6000
Detection
Detection method
ECL
Bands
Specific: 100 kDa
Exposure
10 minute(s) and 0 second(s)
Additional data
Abcam response
The review of the IP of the protein is in review number 41406
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Eleni Petsalaki
Verified customer
投稿 Feb 27 2014