Anti-TSH Receptor/TSH-R 抗体 [SPM223] (ab218328)
Key features and details
- Mouse monoclonal [SPM223] to TSH Receptor/TSH-R
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-TSH Receptor/TSH-R antibody [SPM223]
TSH Receptor/TSH-R 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [SPM223] to TSH Receptor/TSH-R -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human TSH Receptor/TSH-R. (A-Chain protein).
Database link: P16473 -
ポジティブ・コントロール
- IHC-P: Human thyroid carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab218328 was purified from Bioreactor Concentrate by Protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SPM223 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab218328の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT). (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, or 10mM Citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 minutes). |
特記事項 |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, or 10mM Citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 minutes). |
ターゲット情報
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機能
Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5). -
組織特異性
Expressed in the thyroid. -
関連疾患
Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH-independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers).
Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation.
Hypothyroidism, congenital, non-goitrous, 1
Familial gestational hyperthyroidism
Hyperthyroidism, non-autoimmune -
配列類似性
Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
Contains 7 LRR (leucine-rich) repeats. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7253 Human
- Omim: 603372 Human
- SwissProt: P16473 Human
- Unigene: 160411 Human
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別名
- CHNG1 antibody
- hTSHR I antibody
- hTSHRI antibody
see all
画像
データシートおよび資料
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Datasheet download
参考文献 (0)
ab218328 は論文での使用が確認できていません。