Anti-TSH Receptor/TSH-R 抗体 [A7] (ab6044)
Key features and details
- Mouse monoclonal [A7] to TSH Receptor/TSH-R
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-TSH Receptor/TSH-R antibody [A7]
TSH Receptor/TSH-R 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [A7] to TSH Receptor/TSH-R -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Fusion protein corresponding to Human TSH Receptor/TSH-R aa 402-415 (C terminal).
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エピトープ
The murine monoclonal antibody A7 is specific for residues 402-415 of the human TSH receptor. This epitope is localized at the extreme carboxyl terminal of the extracellular domain of the TSH receptor, a region that may be masked from the surface of native TSH receptor. -
ポジティブ・コントロール
- ICC/IF: HEK-293 cells. Flow Cyt: HeLa cells.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituents: PBS, 0.81% Sodium chloride, 0.16% Sodium phosphate, 0.02% Potassium chloride, 0.04% Potassium phosphate -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
A7 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab6044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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ICC/IF | Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5). -
組織特異性
Expressed in the thyroid. -
関連疾患
Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH-independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers).
Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation.
Hypothyroidism, congenital, non-goitrous, 1
Familial gestational hyperthyroidism
Hyperthyroidism, non-autoimmune -
配列類似性
Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
Contains 7 LRR (leucine-rich) repeats. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7253 Human
- Omim: 603372 Human
- SwissProt: P16473 Human
- Unigene: 160411 Human
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別名
- CHNG1 antibody
- hTSHR I antibody
- hTSHRI antibody
see all
画像
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ICC/IF image of ab6044 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6044, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Overlay histogram showing HeLa cells stained with ab6044 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6044, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91633, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
プロトコール
参考文献 (4)
ab6044 は 4 報の論文で使用されています。
- Arauchi A et al. Functional Thyroid Follicular Cells Differentiation from Human-Induced Pluripotent Stem Cells in Suspension Culture. Front Endocrinol (Lausanne) 8:103 (2017). PubMed: 28588551
- Dorris ER et al. Pluripotency markers are differentially induced by MEK inhibition in thyroid and melanoma BRAFV600E cell lines. Cancer Biol Ther 17:526-42 (2016). WB . PubMed: 26828826
- Morgenthaler NG et al. Direct binding of thyrotropin receptor autoantibody to in vitro translated thyrotropin receptor: a comparison to radioreceptor assay and thyroid stimulating bioassay. Thyroid 9:466-75 (1999). PubMed: 10365678
- Nicholson LB et al. Monoclonal antibodies to the human TSH receptor: epitope mapping and binding to the native receptor on the basolateral plasma membrane of thyroid follicular cells. J Mol Endocrinol 16:159-70 (1996). PubMed: 9156519