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  1. Link

    tsh-beta-antibody-11e4-ab6069.pdf

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Signal Transduction Growth Factors/Hormones Hormones
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Anti-TSH beta 抗体 [11E4] (ab6069)

  • Datasheet
  • SDS
Submit a review Q&A (6)

Product price, shipping and contact information

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Mouse monoclonal [11E4] to TSH beta
  • Suitable for: Sandwich ELISA
  • Reacts with: Human
  • Isotype: IgG1

こちらの製品もご検討ください

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関連製品

製品の概要

  • 製品名

    Anti-TSH beta antibody [11E4]
    TSH beta 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [11E4] to TSH beta
  • 由来種

    Mouse
  • 特異性

    This antibody specifically recognizes ß-subunit of human TSH.
  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human TSH beta. Full length native protein (purified) from human pituitary gland.

  • 特記事項

    This antibody (capture) is ELISA paired with ab6070 (conjugate).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • 特記事項(精製)

    Purity is tested by electrophoresis.
  • ポリ/モノ

    モノクローナル
  • クローン名

    11E4
  • ミエローマ

    Sp2/0
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Endocrine system
    • Thyroid axis
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6069の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
特記事項
追加情報
sELISA: Use at an assay dependent dilution.
Can be paired for ELISA with Mouse monoclonal [7G12] to TSH (ab6070).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

  • 関連性

    Various hormones are secreted from the anterior pituitary during development and growth. Thyroid Stimulating Hormone (TSH) is secreted from the anterior pituitary and controls thyroid structure and metabolism
  • 細胞内局在

    Secreted
  • 参照データベース

    • Entrez Gene: 7252 Human
    • Omim: 188540 Human
    • SwissProt: P01222 Human
    • 別名

      • CHNG4 antibody
      • Thyroid stimulating hormone beta subunit antibody
      • Thyroid stimulating hormone, beta precursor antibody
      • Thyrotropin beta subunit antibody
      • TSHB antibody
      see all

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (0)

    ab6069 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6069 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Question

    Inquiry: Will this TSH protein (Active) (ab110682) work as an antigen with the primary antibodies you sell? Specifically mouse monoclonal anti-human TSH antibodies Ab6069, Ab6071 and Ab24921? Thanks!

    Read More

    Abcam community

    Verified customer

    Asked on May 08 2012

    Answer

    Thank you for your enquiry.

    The TSH protein ab110682 is purified human protein. So in theory it should be detected by any antibody which will cross react withhuman TSH. Although please make sure the antibody is also tested in the application of interest.

    I hope this is helpful. Please contact us again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on May 08 2012

    Question

    Quick question that I think I asked you before, but I can't remember what you said. Does Abcam have a "positive control antigen" to test these antibodies with? I am using Thyrotropic hormone from human pituitary from Sigma-Aldrich product number T9265. I contacted Sigma just to make sure there wasn't something weird about this. They did not have specifications on its use in immunoassays and they recommended asking the antibody manufacturer for a positive control. Do you have a TSH antigen that you can recommend?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 04 2012

    Answer

    It is definitely my pleasure to help you with these issues. I look forward to hearing how the new product works in your experiments.

    We do not have the specific antigen used to create these products available on our catalog at this time. However we do offer a full length purified nativeTSH protein which is in its active form. That product number is AB110682 and can be found at the following link:

    https://www.abcam.com/TSH-protein-Active-ab110682.html

    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    Abcam Scientific Support

    Answered on Apr 04 2012

    Question

    sELISA in mouse and rat for TSH, FSH and Luteinizing Hormone
    pairs:
    Luteinizing Hormone: ab72837 (mouse), ab72838 (mouse) - test in mouse, rat
    FSH: ab21011 (mouse, capture), ab30543 (HRP)- test in sELISA, mouse, rat
    TSH: ab6070 (HRP), ab6069 - test in mouse, rat
    send info on proteins for standard curve and secondary Abs (anti-mouse IgG) if required
    Luteinizing Hormone: none
    FSH: ab51888 (human) - test in sELISA
    TSH: ab110682 (human) - test in sELISA
    ab98799 - donkey anti-mouse IgG, preabsorbed, HRP
    ab98808 - goat anti-mouse IgG, preabsorbed, HRP

    Read More

    Abcam community

    Verified customer

    Asked on Mar 08 2012

    Answer

    Thank you for your phone call. I am sending you the following discount codes for trying the antibodies in sELISA and mouse/rat. As for the proteins, I have found the following:

    Luteinizing Hormone: none
    FSH: ab51888 (human) - test in sELISA
    TSH: ab110682 (human) - test in sELISA

    Since the FSH and TSH proteins have not yet been tested in sELISA, I am sending you also discount codes for these proteins (see below). Unfortunately, we do not carry the Luteinizing Hormone protein.

    In terms of secondary antibodies, you would only need one for the Luteinizing Hormone sELISA as none of these antibodies has an HRP label. The other antibody pairs for TSH and FSH contain an HRP-labeled primary antibody.

    The suggested secondary antibodies are:
    ab98799 - donkey anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98799.html)
    ab98808 - goat anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98808.html)

    Testing Discount Codes:

    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in sELISA, mouse, rat
    DISCOUNT CODE: xxx-test in sELISA, mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat

    DISCOUNT CODE: xxx ab51888 (human FSH protein) - test in sELISA
    DISCOUNT CODE: xxx ab110682 (human TSH protein) - test in sELISA

    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test thes antibodies and proteins in sELISA and nouse/rat. Each code will give you 1 free PRIMARY ANTIBODY or PROTEIN before the expiration date. To redeem this offer, please submit each an Abreview for sELISA and mouse/rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Please also remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    Answered on Mar 08 2012

    Question

    Not seeing signal when ab24921 is used to capture TSH from solution. What kind of buffer should be used for the coating and what should the pH be?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 22 2012

    Answer

    Thank you for your call this morning and for your patience.

    There is an error on the pdf form of our sandwich ELISA protocol, and the carbonate buffer should be around 9.6. I've looked through several protocols, and most recommend either a pH 9.6 carbonate buffer or a 7.4 PBS solution for coating. I think that the buffer you are currently using is fine, and I apologize for the mistake on the protocol.

    It's possible that a higher concentration of antibody or antigen will help increase the signal. Could you send me some more details about the protocol and results that you've seen, and I will see if there are any suggestions or explanations?

    I look forward to hearing from you. Please let me know if there is anything else that I can do for you and I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Feb 22 2012

    Question

    What is the ELISA kit that uses this matched pair? How do we convert international units to picograms?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 20 2012

    Answer

    Thank you for contacting us.

    I have have the chance to look over each of the TSH ELISA kits. Unfortunately it appears that none of the currently offered kits uses ab6069 or ab24921. It may be that the particular product that was recommended to you if no longer offered at Abcam.

    Each of our kits is covered by our Abpromise and is 100% guaranteed to work should they be used in a manner consistent with what we have published in the datasheet. However,should you to perform a sandwichELISA using ab6069 I have found that the the matched pair for ab6069 is actually ab6070 and not ab24921. Again, if you decide to purchase this pair for use in sandwich ELISA they will be covered by our Abpromise.

    I was able to find the conversion factor for IU to SI for TSH, that factor is 1.0 and may be found at the following website:

    http://www.questdiagnostics.com/hcp/intguide/SI_Units.pdf

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 20 2012

    Question

    Are these two antibodies cross-reactive with FSH, LH, or HCG?  We were looking for antibodies to the beta subunit, but we may try the ones you suggested if there is no problem with cross-reactivity.  Thank you for checking on it for me!  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 12 2011

    Answer

    Our antibody ab24921 does not react with LH, FSH or HCG, however it is unclear which subunit of the TSH protein this antibody is directed against. On the other hand we do know that ab6069 is specific to the beta subunit of TSH but there is no further information at this time if the antibody shows any cross-reactivity with LH, FSH or HCG.

    Read More

    Abcam Scientific Support

    Answered on Oct 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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