Anti-Tryptophanyl tRNA synthetase/WRS 抗体 [EPR3423] (ab109213)
![Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)](https://www.abcam.co.jp/ps/products/109/ab109213/Images/ab109213-121842-anti-tryptophanyl-trna-synthetase-wrs-antibody-epr3423-western-blot.jpg "Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3423] to Tryptophanyl tRNA synthetase/WRS
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423]
Tryptophanyl tRNA synthetase/WRS 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3423] to Tryptophanyl tRNA synthetase/WRS -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC or IP -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa, JAR, 293T and L6 cell lysates; Human breast tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3423 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109213の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa. |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
追加情報
Is unsuitable for ICC or IP.
ターゲット情報
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機能
Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. -
配列類似性
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 WHEP-TRS domain. -
翻訳後修飾
Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 7453 Human
- Entrez Gene: 22375 Mouse
- Entrez Gene: 314442 Rat
- Omim: 191050 Human
- SwissProt: P23381 Human
- SwissProt: P32921 Mouse
- SwissProt: Q6P7B0 Rat
- Unigene: 497599 Human
see all -
別名
- Gamma 2 antibody
- GAMMA-2 antibody
- Gamma2 antibody
see all
画像
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Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)All lanes : Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : 293T cell lysate
Lane 4 : L6 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 53 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)](https://www.abcam.co.jp/ps/products/109/ab109213/Images/ab109213-121841-anti-tryptophanyl-trna-synthetase-wrs-antibody-epr3423-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)ab109213, at 1/500 dilution, staining Tryptophanyl tRNA synthetase/WRS in paraffin-embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)](https://www.abcam.co.jp/ps/products/109/ab109213/Images/ab109213-3-benefits-of-recombinant-antibodies.png)
Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab109213 は論文での使用が確認できていません。
