Anti-Tryptophan Hydroxylase/TPH 抗体 [EP1311Y] (ab52954)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1311Y] to Tryptophan Hydroxylase/TPH
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y]
Tryptophan Hydroxylase/TPH 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1311Y] to Tryptophan Hydroxylase/TPH -
由来種
Rabbit -
特異性
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human small intestine and pituitary tissue lysate. Mouse brain tissue lysate. Recombinant full length mouse Tph1 protein with C-terminal MYC/DDK tag. N-His tagged human Tryptophan Hydroxylase/TPH (aa 105 to 420) recombinant protein
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1311Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52954の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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IHC-P | (2) |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. For the unpurified format use at 1/50 dilution |
特記事項 |
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WB
1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. For the unpurified format use at 1/50 dilution |
ターゲット情報
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組織特異性
Isoform 2 seems to be less widely expressed than isoform 1. -
パスウェイ
Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. -
配列類似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain. - Information by UniProt
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参照データベース
- Entrez Gene: 7166 Human
- Entrez Gene: 21990 Mouse
- Omim: 191060 Human
- SwissProt: P17752 Human
- SwissProt: P17532 Mouse
- Unigene: 591999 Human
- Unigene: 248684 Mouse
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別名
- Indoleacetic acid 5 hydroxylase antibody
- L tryptophan hydroxylase antibody
- MGC119994 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling Tryptophan Hydroxylase/TPH with Purified ab52954 at 1:2000 dilution (1.191 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Lane 1 : Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution
Lane 2 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) at 1/1000 dilution
All lanes : Full-length Tph1 mouse recombinant protein with C-terminal MYC/DDK tag 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution
Lane 1 : Human small intestine tissue lysate
Lane 2 : Human pituitary tissue lysate
Lane 3 : Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 180 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
TPH1 is mainly expressed in non-neuronal peripheral tissues like pineal gland.
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Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution + N-His tagged human Tryptophan Hydroxylase/TPH (aa 105 to 420) recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (24)
ab52954 は 24 報の論文で使用されています。
- Omoluabi T et al. Novelty-like activation of locus coeruleus protects against deleterious human pretangle tau effects while stress-inducing activation worsens its effects. Alzheimers Dement (N Y) 7:e12231 (2021). PubMed: 35005208
- Yi G et al. A study of the mechanism of small-molecule soybean-protein-derived peptide supplement to promote sleep in a mouse model. RSC Adv 10:11264-11273 (2020). PubMed: 35495343
- Chen DQ et al. Poricoic acid A as a modulator of TPH-1 expression inhibits renal fibrosis via modulating protein stability of ß-catenin and ß-catenin-mediated transcription. Ther Adv Chronic Dis 11:2040622320962648 (2020). PubMed: 33062239
- Cheng Q et al. [6]-Gingerol Ameliorates Cisplatin-Induced Pica by Regulating the TPH/MAO-A/SERT/5-HT/5-HT3 Receptor System in Rats. Drug Des Devel Ther 14:4085-4099 (2020). PubMed: 33061309
- Chen DQ et al. Identification of serum metabolites associating with chronic kidney disease progression and anti-fibrotic effect of 5-methoxytryptophan. Nat Commun 10:1476 (2019). PubMed: 30931940