Trypsin Antigen Retrieval Solution (ab970)
製品の概要
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製品名
Trypsin Antigen Retrieval Solution -
アプリケーション
適用あり: IHC-Pmore details -
特記事項
Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970). Contains colored pH indicator for easy pH confirmation.
Protocol Notes: 1) Deparaffinize tissues and hydrate to water. If necessary perform a hydrogen peroxide block, wash in water, and rinse in PBS or TBS. The tissue section is ready for trypsin digestion. 2) Combine 1 part trypsin concentrate with 1 part buffer (working solution stable for 5 working days at 2-8ºC). Incubate section for 5-10 minutes at 37°C or 15 minutes at room temperature. Then, rinse well in PBS or TBS buffer and proceed with immunostaining procedure. This product utilises a color-coded pH indicator (rose) for easy identification. The end-user can visually inspect the solution and see that the mixture or buffer is at the proper pH. If the mixed solution is purple, the pH is too high for optimum digestion. If the buffer or trypsin solution turns orange or yellow, the pH is too low for optimum digestion.
Trypsin is a commonly used digestive enzyme. In formalin-fixed, paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining.
Other products for IHC
Other enzymes for antigen retrieval include: Proteinase K Antigen Retrieval Solution ab64220 and Pepsin Solution ab64201.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, Carrier protein -
Concentration information loading...
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IHC 試薬 備考
Trypsin is a commonly used digestive enzyme. In formalin-fixed, paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining. -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab970の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent dilution. Ratio for 25ml concentrated trypsin and 25 ml buffer is 1:1 - 1:3.
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特記事項 |
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IHC-P
Use at an assay dependent dilution. Ratio for 25ml concentrated trypsin and 25 ml buffer is 1:1 - 1:3. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab970 は 15 報の論文で使用されています。
- Wang Y et al. PDGFRa marks distinct perivascular populations with different osteogenic potential within adipose tissue. Stem Cells 38:276-290 (2020). PubMed: 31742801
- Sorkin M et al. Regulation of heterotopic ossification by monocytes in a mouse model of aberrant wound healing. Nat Commun 11:722 (2020). PubMed: 32024825
- Xu J et al. Comparison of skeletal and soft tissue pericytes identifies CXCR4+ bone forming mural cells in human tissues. Bone Res 8:22 (2020). PubMed: 32509378
- Shen AH et al. Prophylactic treatment with transdermal deferoxamine mitigates radiation-induced skin fibrosis. Sci Rep 10:12346 (2020). PubMed: 32704071
- Kim HS et al. Morphological characteristics of vasculogenic mimicry and its correlation with EphA2 expression in gastric adenocarcinoma. Sci Rep 9:3414 (2019). PubMed: 30833656